Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants

Affiliations

• ¹ National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China.
• ² National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
• ³ College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
• ⁴ National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093-0116, USA. Electronic address: yundezhao@ucsd.edu.