Trunk surface agarwood-inducing technique with Rigidoporus vinctus: An efficient novel method for agarwood production

PLoS One. 2018 Jun 1;13(6):e0198111. doi: 10.1371/journal.pone.0198111. eCollection 2018.

Abstract

Only when Aquilaria spp. or Gyrinops spp. trees are wounded, due to insect attack, or microbial invasion, agarwood can be successfully induced. In the present study, a fungus which can induce agarwood formation efficiently was isolated and a suitable method for its application to induce agarwood formation was developed. Rigidoporus vinctus was isolated from the inner layers from infectious A. sinensis trees. When the fermentation liquid of fungi inoculated back to A. sinensis tree, agarwood was found to be induced. In addition, a novel method called trunk surface agarwood-inducing technique (Agar-Sit) was developed to produce agarwood with R. vinctus. The alcohol soluble extract content of the agarwood, up to 38.9%, far higher than the requirement (10%) in Chinese Pharmacopoeia and the six characteristic compounds of agarwood used as Chinese Medicinal Materials were all detected. Their relative percentages of the sesquiterpenes in the essential oil were 22.76%. This is the first report of the Agar-Sit and also the application of R. vinctus in agarwood induction. According to the results, when the combination of Agar-Sit and R. vinctus is used agarwood can be induced with high yield and good quality.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Drugs, Chinese Herbal / analysis
  • Drugs, Chinese Herbal / isolation & purification
  • Fermentation
  • Fungi / isolation & purification
  • Oils, Volatile / chemistry
  • Oils, Volatile / isolation & purification
  • Plant Stems / metabolism
  • Plant Stems / microbiology
  • Polyporales / chemistry
  • Polyporales / isolation & purification
  • Polyporales / physiology*
  • Resins, Plant / chemistry
  • Resins, Plant / metabolism*
  • Sesquiterpenes / analysis
  • Thymelaeaceae / metabolism
  • Thymelaeaceae / microbiology*

Substances

  • Drugs, Chinese Herbal
  • Oils, Volatile
  • Resins, Plant
  • Sesquiterpenes

Grants and funding

This study was funded by the National Natural Science Foundation of China (grant numbers 81673549, 81303312 and 81403055) (http://www.nsfc.gov.cn/); Major Science And Technology Plan Project of Hainan Province (grant number ZDKJ2016004) (http://dost.hainan.gov.cn/); Medical and Health Science and Technology Innovation Project (Major Collaborative Innovation Project) (grant number 2016-I2M-2-003); Science & Technology Program of Hainan Province (grant number ZDXM2015059). "Ten Thousand Plan” -National High Level Talents Special Support Plan (grant number 99950534) and Natural Science Foundation of Hainan province (grant number 20168369). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.