Cissus quadrangularis extract (CQR-300) inhibits lipid accumulation by downregulating adipogenesis and lipogenesis in 3T3-L1 cells

Hae Jin Lee; Bao Le; Dong-Ryung Lee; Bong-Keun Choi; Seung Hwan Yang

doi:10.1016/j.toxrep.2018.02.008

Cissus quadrangularis extract (CQR-300) inhibits lipid accumulation by downregulating adipogenesis and lipogenesis in 3T3-L1 cells

Toxicol Rep. 2018 Mar 30:5:608-614. doi: 10.1016/j.toxrep.2018.02.008. eCollection 2018.

Authors

Hae Jin Lee¹, Bao Le¹, Dong-Ryung Lee², Bong-Keun Choi², Seung Hwan Yang¹

Affiliations

¹ Department of Biotechnology, Chonnam National University, Yeosu 59626, Republic of Korea.
² Nutrapharm Tech, Jungwon-gu, Seongnam, Gyeonggi 13201, Republic of Korea.

Abstract

The objective of this study was to evaluate the anti-obesity activity and the action mechanism of Cissus quadrangularis extracts (CQR-300) in 3T3-L1 adipocytes. Cissus quadrangularis was extracted with hot water, resulting in CQR-300. The anti-obesity activity of CQR-300 in 3T3-L1 adipocytes was examined by Oil-red O staining. Possible mechanisms of CQR-300 in 3T3-L1 adipocytes were determined by real-time PCR and western blot. Treatment with CQR-300 inhibited lipid accumulation without showing cytotoxicity to 3T3-L1 adipocytes. Furthermore, CQR-300 decreased adipogenesis/lipogenesis-related mRNA expression levels of fatty acid binding protein (aP2), fatty acid synthase (FAS), lipoprotein lipase (LPL), stearoyl-CoA desaturase-1 (SCD-1), and acetyl-CoA carboxylase (ACC). CQR-300 also down-regulated expression levels of adipogenesis/lipogenesis-associated proteins, including peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), sterol regulatory element binding protein-1c (SREBP-1c), and FAS. It's also up-regulated the expression level of phosphorylated-AMPK (p-AMPK). Collectively, these results suggested that CQR-300 might have an anti-obesity effect by its ability to decrease expression levels of adipogenesis/lipogenesis-related genes and proteins.

Keywords: ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein kinase; Adipocytes; Adipogenesis; Anti-obesity; BCS, bovine calf serum; C/EBPα, CCAAT/enhancer-binding protein α; CQR-300, Cissus quadrangularis extract; Cissus quadrangularis extract (CQR-300); DMEM, Dulbecco’s modified Eagle’s medium; FAS, fatty acid synthase; FAS-α, fatty-acid synthase; FBS, fetal bovine serum; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LPL, lipoprotein lipase; Lipogenesis; MDI, medium dependent interface; MTT, 3-(4, 5-dimetylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; ORO, Oil-red O; PPARγ, peroxisome proliferator-activated receptor γ; RIPD, radioimmunoprecipitation assay buffer; SCD-1, stearoyl-CoA desaturase-1; SREBP-1c, sterol regulatory element binding protein-1c; TG, triglycerides; aP2, fatty acid binding protein (aP2); p-AMPK, phosphorylated-AMPK.