We report the baseline usability of a novel web-based application, the Population Health Record (PopHR), designed to facilitate the effective use of population health information by public health professionals and to support evidence-based decision-making. The usability test was conducted with ten potential users who each completed eight tasks using the PopHR system. Participant responses were recorded, including timestamps for each data entry. Overall, the task completion rate was 96% while the success rate was 88%. The average time-on-task was 3.11 minutes, with more time spent on tasks requiring a user to stratify data along multiple dimensions, such as age, sex, or geographical region. Usability scores indicated that the current version of PopHR has good usability. Potential improvements identified included adding supporting information, offering different visualizations, and enhancing system stability. These findings are examples of addressable usability problems encountered in developing a population health record system.