Mussel-inspired dual-functional PEG hydrogel inducing mineralization and inhibiting infection in maxillary bone reconstruction

Huiyong Xu; Ge Zhang; Kaige Xu; Leyu Wang; Lei Yu; Malcolm M Q Xing; Xiaozhong Qiu

doi:10.1016/j.msec.2018.04.066

Mussel-inspired dual-functional PEG hydrogel inducing mineralization and inhibiting infection in maxillary bone reconstruction

Mater Sci Eng C Mater Biol Appl. 2018 Sep 1:90:379-386. doi: 10.1016/j.msec.2018.04.066. Epub 2018 Apr 23.

Authors

Huiyong Xu¹, Ge Zhang², Kaige Xu³, Leyu Wang², Lei Yu², Malcolm M Q Xing⁴, Xiaozhong Qiu⁵

Affiliations

¹ Department of Stomatology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Nanfang Hospital, Southern Medical University, Guangdong, Guangzhou 510515, China; Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangdong, Guangzhou 510515, China.
² Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangdong, Guangzhou 510515, China.
³ Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
⁴ Department of Plastic and Aesthetic Surgery, Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong Province 510515, China; Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. Electronic address: malcolm.xing@umanitoba.ca.
⁵ Department of Stomatology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Nanfang Hospital, Southern Medical University, Guangdong, Guangzhou 510515, China; Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangdong, Guangzhou 510515, China. Electronic address: qqiuxzh@163.com.

PMID: 29853103
DOI: 10.1016/j.msec.2018.04.066

Abstract

Infection compromises healing process after bone fracture. An anti-bacterial bone graft synthesized from polymer and mineralization components is becoming preferable for its accessibility and low cost and tunable chem-physic properties. In this study, mussel-inspired polydopamine (PDA) was used to synthesize in-situ silver nanoparticles (AgNPs) and mineralization on polyethylene hydrogel (PEG). With dual functions of anti-bacteria and graft mineralization, we found the hydrogel (AgNPs/PDA) promoted bone generation and show significant antibacterial activity. Specifically, the gel upregulated the expression of osteogenic genes of bone sialoprotein gene, alkaline phosphatase, osteocalcin and runt-related transcription factor 2. It also significantly inhibited the growth of Staphylococcus aureus and Escherichia coli. In vivo the AgNPs/PDA gel could repair maxillary bone defect efficiently.

Keywords: Anti-bacterial; Maxillary bone implant; Mussel-inspired coating; Nano‑silver; Osteogenic hydrogel.

MeSH terms

Animals
Anti-Bacterial Agents / adverse effects
Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / pharmacology
Cell Line
Cell Survival / drug effects
Coated Materials, Biocompatible / adverse effects
Coated Materials, Biocompatible / chemistry
Coated Materials, Biocompatible / pharmacology
Male
Metal Nanoparticles / adverse effects
Metal Nanoparticles / chemistry*
Mice
Microbial Sensitivity Tests
Microscopy, Atomic Force
Polymers / chemistry*
Rats
Rats, Sprague-Dawley
Silver / chemistry*

Substances

Anti-Bacterial Agents
Coated Materials, Biocompatible
Polymers
Silver