Proteomic profiling of 24-epibrassinolide-induced chilling tolerance in harvested banana fruit

Abstract

The mechanism of 24-epibrassinolide (EBR)-induced chilling tolerance in harvested banana fruit was investigated. Results showed that EBR pretreatment remarkably suppressed the development of chilling injury (CI) in harvested banana fruit during 12 days of cold storage at 8 °C, as indicated by lower CI index in treated fruit. Physiological measurements exhibited that EBR treatment reduced the relative electrolyte leakage and malondialdehyde (MDA) content while increased the chlorophyll fluorescence (Fv/Fm), total soluble solids (TSS) and ratio of TSS and titratable acidity. Furthermore, the differentially accumulated proteins of banana fruit in response to EBR and cold treatment were investigated by employing gel-based proteomic in combination with MALDI-TOF-TOF MS and LC-ESI-MS/MS analyses. There were fifty five protein spots to be successfully identified. Notably, most of up-regulated proteins by EBR treatment were related to energy biosynthesis, stress response and cell wall modification. In contrast, proteins involved in protein degradation and energy consumption were down-regulated by EBR treatment. These results suggest that EBR treatment could enhance the defense ability, promote the synthesis and utilization of energy, as well as maintain the protein function via enhancing protein biosynthesis and inhibiting protein degradation, consequently contributing to improvement of cold tolerance in harvested banana fruit.

Significance: To extend our understanding of chilling injury (CI) of harvested banana fruit, we reported the effect of 24-epibrassinolide (EBR) on CI of banana fruit when stored at 8 °C. It was the first report on the comprehensive proteomic analysis of banana fruit in response to EBR treatment at low temperature. EBR pretreatment significantly reduced CI in harvested banana fruit. Fifty five protein spots were successfully identified. Notably, the most of up-regulated proteins by EBR treatment were related to energy biosynthesis, stress response and cell wall modification. In contrast, proteins involved in protein degradation and energy consumption were down-regulated. These results suggest that exogenous EBR treatment could enhance the defense ability and maintain high energy status. Meanwhile, EBR treatment maintained protein function via enhancing protein biosynthesis and inhibiting protein degradation. These results may help us to understand the molecular mechanism of the chilling tolerance induced by EBR treatment and broaden the current knowledge of the mechanism of CI of harvested banana fruit.

Keywords: Banana; Low temperature; Plant hormone; Protein function; Redox stress.