Abstract
We have shown that in the human peripheral blood cells, the innate immunity protein Tag7 can activate a subpopulation of CD3+CD4+CD25+ cells, which have antitumor activity. These cells can induce lysis of HLA-negative tumor cell lines. The Hsp70 stress molecule on the surface of the tumor cells is used as a recognition target, while the Tag7 protein on the lymphocyte membrane acts as a receptor for Hsp70. We have also demonstrated that this subpopulation of the CD4+CD25+ cells is CD127 positive and hence is not the Treg cells. Our data suggest that this subpopulation of cells is identical to the CD4+CD25+ lymphocytes, which are activated in the leukocyte pool by the IL-2 cytokine.
MeSH terms
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Animals
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Antigens, Neoplasm / immunology
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CD3 Complex / metabolism
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CD4-Positive T-Lymphocytes / immunology*
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Cell Differentiation
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Cell Proliferation
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Cytokines / metabolism*
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Cytotoxicity, Immunologic
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HSP70 Heat-Shock Proteins / metabolism*
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HeLa Cells
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Humans
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Immunity, Innate
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Interleukin-2 / metabolism
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Interleukin-2 Receptor alpha Subunit / metabolism
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Interleukin-7 Receptor alpha Subunit / metabolism
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K562 Cells
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Mice
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Neoplasms, Experimental / immunology*
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T-Lymphocyte Subsets / immunology*
Substances
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Antigens, Neoplasm
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CD3 Complex
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Cytokines
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HSP70 Heat-Shock Proteins
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Interleukin-2
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Interleukin-2 Receptor alpha Subunit
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Interleukin-7 Receptor alpha Subunit
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PGLYRP1 protein, human