Neuropeptides and lymphocyte populations in the porcine ileum and ileocecal lymph nodes during postnatal life

PLoS One. 2018 May 29;13(5):e0196458. doi: 10.1371/journal.pone.0196458. eCollection 2018.

Abstract

The maturation-related changes in the concentrations of galanin (Gal), vasoactive intestinal polypeptide (VIP), substance P (SP) and somatostatin (Som), as well as in subpopulations of lymphocytes expressing antigens CD2 (lymphocytes T), CD4 (T helper), CD8 (T cytotoxic), CD21 (B lymphocytes), CD5-/CD8+ (NK cells) and TCRgamma/delta (gut mucosal/intraepitelial cells) were studied in the ileal Peyer's patches and ileo-cecal lymph nodes in female pigs aged 3 days, 2 weeks, 4 weeks and 4 months. As regards neuropeptide concentrations statistically significant changes in the ileum and lymph nodes were found only in case of Gal and VIP. The concentrations of neuropeptides were significantly higher only in new-born animals. As regards the changes in subpopulations of lymphocytes, statistically significant changes were noticed only in 4-months old animals and were dealing only with CD2+ and TCRgamma/delta cells in the ileum as well as CD4+, CD8+, CD21+ and TCRgamma/delta in lymph nodes. The highest number of CD8+, CD21+ and TCRgamma/delta lymphocytes occurred in 4-months old animals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Cecum / growth & development
  • Cecum / immunology
  • Cecum / metabolism
  • Female
  • Galanin / metabolism
  • Ileum / growth & development
  • Ileum / immunology*
  • Ileum / metabolism*
  • Immunohistochemistry
  • Lymph Nodes / immunology
  • Lymph Nodes / metabolism
  • Lymphocyte Subsets / immunology*
  • Neuropeptides / metabolism*
  • Peyer's Patches / immunology
  • Peyer's Patches / metabolism
  • Somatostatin / metabolism
  • Substance P / metabolism
  • Sus scrofa
  • Vasoactive Intestinal Peptide / metabolism

Substances

  • Neuropeptides
  • Substance P
  • Vasoactive Intestinal Peptide
  • Somatostatin
  • Galanin

Grants and funding

Publication supported by KNOW (Leading National Research Centre) Scientific Consortium "Healthy Animal - Safe Food", decision of Ministry of Science and Higher Education No. 05- 1/KNOW2/2015.