Development of glycosynthases with broad glycan specificity for the efficient glyco-remodeling of antibodies

Chem Commun (Camb). 2018 Jun 12;54(48):6161-6164. doi: 10.1039/c8cc03384f.

Abstract

The first systematic investigation of the effect of high mannose, hybrid, and bi- and tri-antennary complex type glycans on the effector functions of antibodies was achieved by the discovery of novel Endo-S2 mutants generated by site-directed mutagenesis as glycosynthases with broad substrate specificity.

MeSH terms

  • Antibodies / chemistry*
  • Antibodies / metabolism
  • Glycoside Hydrolases / genetics
  • Glycosylation
  • Glycosyltransferases / chemistry*
  • Glycosyltransferases / genetics
  • Mutagenesis, Site-Directed
  • Polysaccharides / chemistry*
  • Protein Engineering
  • Receptors, IgG / metabolism
  • Streptococcus pyogenes / enzymology
  • Structure-Activity Relationship
  • Substrate Specificity

Substances

  • Antibodies
  • Polysaccharides
  • Receptors, IgG
  • Glycosyltransferases
  • Glycoside Hydrolases