Rapid and sensitive double-label based immunochromatographic assay for zearalenone detection in cereals

Electrophoresis. 2018 Aug;39(16):2125-2130. doi: 10.1002/elps.201800055.

Abstract

A double-label immunochromatographic based assay (DL-ICA) was developed to monitor zearalenone (ZEN) levels in cereals, based on Eu3+ nanoparticles (EuNP). The DL-ICA exhibited excellent sensitivity, reliability and selectivity in real samples. It showed low limits of detection (0.21-0.25 μg/kg) and broad analytical ranges (up to 120 μg/kg). The total analytical time, including sample preparation and DL-ICA execution, was reduced by 15 min compared with HPLC. The recovery rates ranged from 95.0-118.4%, with relative standard deviations (RSD) <11.6%. Inter- and intra-day validations were assessed, recovery rates of 89.3-106.9% and RSD of 2.3-9.7% were obtained, suggesting considerable stability and reliability for the assay. An excellent correlation was observed between DL-ICA and a reference HPLC method (R2 = 0.9899). Compared to current immunoassays, the current DL-ICA is inexpensive, highly sensitive, and rapid. Therefore, DL-ICA constitutes a novel tool for monitoring mycotoxins in food and feed to ensure safety.

Keywords: Cereals; Double-label immunochromatographic assay; Quantitative; Zearalenone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Edible Grain / chemistry*
  • Food Contamination / analysis*
  • Immunoassay / economics
  • Immunoassay / methods*
  • Immunoassay / standards
  • Limit of Detection
  • Mycotoxins / analysis
  • Reproducibility of Results
  • Time Factors
  • Zearalenone / analysis*

Substances

  • Mycotoxins
  • Zearalenone