Investigation of lead bioimmobilization and transformation by Penicillium oxalicum SL2

Binhui Ye; Yating Luo; Junyu He; Lijuan Sun; Bibo Long; Qinglin Liu; Xiaofeng Yuan; Peibin Dai; Jiyan Shi

doi:10.1016/j.biortech.2018.05.066

Investigation of lead bioimmobilization and transformation by Penicillium oxalicum SL2

Bioresour Technol. 2018 Sep:264:206-210. doi: 10.1016/j.biortech.2018.05.066. Epub 2018 May 18.

Authors

Binhui Ye¹, Yating Luo², Junyu He³, Lijuan Sun⁴, Bibo Long², Qinglin Liu², Xiaofeng Yuan⁵, Peibin Dai⁶, Jiyan Shi⁷

Affiliations

¹ Department of Environmental Engineering, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China; MOE Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China.
² Department of Environmental Engineering, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China.
³ Ocean College, Zhejiang University, Zhoushan, 316021, China.
⁴ Institute of ECO-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.
⁵ College of Life Science, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
⁶ Department of Applied Engineering, Zhejiang Economic and Trade Polytechnic, Hangzhou, 310018, China.
⁷ Department of Environmental Engineering, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China; MOE Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China. Electronic address: shijiyan@zju.edu.cn.

PMID: 29803812
DOI: 10.1016/j.biortech.2018.05.066

Abstract

Fungi Penicillium oxalicum SL2 was applied for Pb²⁺ bioremediation in aqueous solution in this study. After 7 days of incubation at different initial concentrations of Pb²⁺ (0, 100, 500 and 2500 mg L^-1), most of Pb²⁺ were removed (90, 98.3, and 86.2%), the maximum Pb content in mycelium reached about 155.6 mg g^-1 dw. Meanwhile, the formation of extracellular secondary minerals and intracellular Pb-complex were observed and identified, the speciation of Pb in mycelium was also detected by X-ray absorption near-edge structure (XANES) spectroscopy, i.e., Pb-oxalate, Pb-citrate, Pb-hydrogen phosphate and Pb-glutathione analogues. In addition, content of glutathione and oxidized glutathione was increased under the exposure of Pb²⁺, which implied that glutathione might play a key role in Pb immobilization and detoxification in P. oxalicum SL2. This study elucidated partial mechanisms of Pb immobilization and speciation transformation of this strain, providing an alternative biomaterial in the bioremediation of Pb-contaminated wastewater.

Keywords: Bioimmobilization; Glutathione; Pb; Penicillium oxalicum SL2; Transformation.

MeSH terms

Biotransformation
Environmental Pollutants / isolation & purification*
Lead / isolation & purification*
Minerals
Penicillium*
Phosphates
X-Ray Absorption Spectroscopy

Substances

Environmental Pollutants
Minerals
Phosphates
Lead