[12]aneN3-based lipid with naphthalimide moiety for enhanced gene transfection efficiency

Yong-Guang Gao; Uzair Alam; Ai-Xiang Ding; Quan Tang; Zheng-Li Tan; You-Di Shi; Zhong-Lin Lu; Ai-Rong Qian

doi:10.1016/j.bioorg.2018.04.018

[12]aneN₃-based lipid with naphthalimide moiety for enhanced gene transfection efficiency

Bioorg Chem. 2018 Sep:79:334-340. doi: 10.1016/j.bioorg.2018.04.018. Epub 2018 May 3.

Authors

Yong-Guang Gao¹, Uzair Alam², Ai-Xiang Ding³, Quan Tang², Zheng-Li Tan², You-Di Shi², Zhong-Lin Lu⁴, Ai-Rong Qian⁵

Affiliations

¹ Laboratory for Bone Metabolism, Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China; Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China.
² Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China.
³ Xinyang Normal University, Xinyang, Henan 464000, China.
⁴ Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Xinjiekouwai Street 19, Beijing 100875, China. Electronic address: luzl@bnu.edu.cn.
⁵ Laboratory for Bone Metabolism, Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China. Electronic address: qianair@nwpu.edu.cn.

PMID: 29803080
DOI: 10.1016/j.bioorg.2018.04.018

Abstract

Three cationic lipids derived from [12]aneN₃ modified with naphthalimide (1a), oleic acid (1b) and octadecylamine (1c) were designed and synthesized. In vitro transfection showed that all these liposomes can deliver plasmid DNA into the tested cell lines. Among these liposomes, 1a gave the best transfection efficiency (TE) in A549 cells, which was higher than that of lipofectamine 2000. More importantly, the TE of 1a was dramatically increased in the presence of 10% serum. These results suggested that 1a might be a promising non-viral gene vector, and also give further insight for developing novel high performance gene delivery agents.

Keywords: Cationic lipids; Naphthalimide; Non-viral vectors; Transfection efficiency; [12]aneN3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell Survival / drug effects
Dose-Response Relationship, Drug
Gene Transfer Techniques*
Humans
Lipids / chemistry*
Lipids / pharmacology
Luciferases / genetics*
Luciferases / metabolism
Molecular Structure
Naphthalimides / chemistry*
Naphthalimides / pharmacology
Structure-Activity Relationship
Transfection

Substances

Lipids
Naphthalimides
Luciferases