Chitosan-functionalized supermagnetic halloysite nanotubes for covalent laccase immobilization

Avinash A Kadam; Jiseon Jang; Seung Cheol Jee; Jung-Suk Sung; Dae Sung Lee

doi:10.1016/j.carbpol.2018.04.046

Chitosan-functionalized supermagnetic halloysite nanotubes for covalent laccase immobilization

Carbohydr Polym. 2018 Aug 15:194:208-216. doi: 10.1016/j.carbpol.2018.04.046. Epub 2018 Apr 14.

Authors

Avinash A Kadam¹, Jiseon Jang², Seung Cheol Jee³, Jung-Suk Sung⁴, Dae Sung Lee⁵

Affiliations

¹ Research Institute of Biotechnology and Medical Converged Science, Dongguk University, Biomedi Campus, Ilsandong-gu, Goyang-si, Gyeonggi-do 10326, Republic of Korea.
² Department of Environmental Engineering, Kyungpook National University, 80 Daehak-ro, Buk-Gu, Daegu 41566, Republic of Korea.
³ Department of Life Sciences, Dongguk University-Ilsan, Biomedi Campus, 32 Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10326, Republic of Korea.
⁴ Department of Life Sciences, Dongguk University-Ilsan, Biomedi Campus, 32 Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10326, Republic of Korea. Electronic address: sungjs@dongguk.edu.
⁵ Department of Environmental Engineering, Kyungpook National University, 80 Daehak-ro, Buk-Gu, Daegu 41566, Republic of Korea. Electronic address: daesung@knu.ac.kr.

PMID: 29801831
DOI: 10.1016/j.carbpol.2018.04.046

Abstract

Halloysite nanotubes (HNTs) were modified with supermagnetic Fe₃O₄ (M-HNTs) and functionalized with chitosan (CTA) (termed as M-HNTs-CTA). Furthermore, M-HNTs-CTA were cross-linked using glutaraldehyde and applied for covalent laccase immobilization (M-HNTs-CTA-Lac). Facile-synthesized modified HNTs were structurally characterized by scanning electron microscopy, high resolution transmission electron microscopy, X-ray photoelectron spectroscopy, and thermogravimetric analyses. M-HNTs-CTA-Lac exhibited 92.74 mg/g of laccase immobilization capacity and 92% of activity recovery. Biochemical properties of M-HNTs-CTA-Lac exhibited higher pH and temperature stabilities, with exceptional reusability capabilities until the 11^th cycle. Moreover, M-HNTs-CTA-Lac exhibited 87% of 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)-mediated Direct Red 80 (DR80) decolorization. By the 11^th cycle, M-HNTs-CTA-Lac exhibited 33% DR80 decolorization. Therefore, M-HNTs-CTA can function as CTA-modified supermagnetic nonreactors for immobilization of biomacromolecules. The investigated M-HNTs-CTA-Lac are thus biocompatible and environment-friendly biocatalysts for degradation of textile waste, such as DR80, and can be rapidly retrieved from aqueous solution by a magnet after decontamination of environmental pollutants.

Keywords: Chitosan; Halloysite nanotubes; Laccase; Laccase-loaded nanotubular reactors; Nanobiocatalysis; Sustainable environmental cleanup.