Characterization of amylose nanoparticles prepared via nanoprecipitation: Influence of chain length distribution

Yanjiao Chang; Jingde Yang; Lili Ren; Jiang Zhou

doi:10.1016/j.carbpol.2018.03.104

Characterization of amylose nanoparticles prepared via nanoprecipitation: Influence of chain length distribution

Carbohydr Polym. 2018 Aug 15:194:154-160. doi: 10.1016/j.carbpol.2018.03.104. Epub 2018 Apr 3.

Authors

Yanjiao Chang¹, Jingde Yang¹, Lili Ren¹, Jiang Zhou²

Affiliations

¹ Key Laboratory of Bionic Engineering (Ministry of Education), College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China.
² Key Laboratory of Bionic Engineering (Ministry of Education), College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China. Electronic address: jiang.zhou@jlu.edu.cn.

PMID: 29801823
DOI: 10.1016/j.carbpol.2018.03.104

Abstract

The influence of chain length distribution of amylose on size and structure of the amylose nanoparticles (ANPs) prepared through nanoprecipitation was investigated. Amylose with different chain length distributions was obtained by β-amylase treating amylose paste for different times and measured by size exclusion chromatography (SEC) and fluorophore-assisted carbohydrate electrophoresis (FACE). ANPs prepared via precipitation were characterized by using dynamic light scattering (DLS), scanning electron microscopy (SEM) and X-ray diffraction (XRD). Results showed that the β-amylase treatments led to decrease in chain length of amylose, and it was the most important factor affecting size of ANPs. When hydrolysis degree of amylose was 52.8%, mean size of ANPs decreased from 206.4 nm to 102.7 nm. All the ANPs displayed a V-type crystalline structure and the effect of amylose chain length on crystallinity of the precipitated ANPs was negligible in the investigated range.

Keywords: Amylose; Chain length distribution; Nanoparticles; Precipitation.