Single Passage of Human Metapneumovirus in LLC-MK2 Cells Does Not Affect Viral Protein-Coding Capacity

Simon Loevenich; Aleksandr Ianevski; Eneli Oitmaa; Denis E Kainov; Marit W Anthonsen

doi:10.1128/genomeA.00440-18

Single Passage of Human Metapneumovirus in LLC-MK2 Cells Does Not Affect Viral Protein-Coding Capacity

Genome Announc. 2018 May 24;6(21):e00440-18. doi: 10.1128/genomeA.00440-18.

Authors

Simon Loevenich¹, Aleksandr Ianevski¹, Eneli Oitmaa², Denis E Kainov^{1

3}, Marit W Anthonsen⁴

Affiliations

¹ Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway.
² Institute of Genomics, University of Tartu, Tartu, Estonia.
³ Institute of Technology, University of Tartu, Tartu, Estonia.
⁴ Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway marit.w.anthonsen@ntnu.no.

Abstract

Here, we report the complete genome sequences of human metapneumovirus (HMPV) prior to and after passaging in LLC-MK2 cells. Paired comparisons of the 13,335-nucleotide genomes revealed that the virus acquired the T10736C transition in its genome, which did not affect the amino acid sequences of HMPV proteins.