[Expression of Has-miR-93-5p in laryngeal squamous cell carcinoma and its clinical significance]

Abstract

Objective:To investigate the expression of has-miR-93-5p on human laryngeal squamous cell carcinoma and the influence on malignant phenotype of Hep-2 cell.Method:The expression of has-miR-93-5p of paraffin samples in LSCC was determined by looped-primer Real-time PCR, and the relationship between the expression and the clinical pathological parameters was analysed. The has-miR-93-5p Inhibitor sequence was transfected into Hep-2 cells as the Inhibitor group. Using the MTS assay, Edu and colony formation assay to investigate the change of cell viability,proliferation and clone formation ability after transfection. Transwell invasion assay was used to detect the changes of cell migration and invasion ability. Flow cytometry was used to detect the changes of cell cycle and apoptosis.Result:The relative expression of has-miR-93-5p in LSCC was 11.148±1.141,which was higher than in normal tissues of adjacent to carcinoma1(985±4.547)（P <0.01）.The constituent ratio of has-miR-93-5p high expression in the group of low differentiation, T3+T4 and lymphatic metastasis was 69.8%、76.5%and 89.5%,which was higher than the group of high differentiation,T1+T2 and nonlymphatic metastasis respectively（P <0.05）, Inhibition the expression of has-miR-93-5p in vitro in Hep-2 cells could obviously inhibit the cell vitality, proliferation, clone, migration, and invasion ability, also could retardant the cells in G2/M phase, and promote its apoptosis.Conclusion: has-miR-93-5p might be an important molecule in pathogenesis of laryngeal squamous cell carcinoma. It could inhibit malignant phenotype of laryngeal squamous cancer cells when has-miR-93-5p expressionwas suppressed in vitro.

Keywords: has-miR-93-5p; invasion and metastasis; laryngeal carcinoma; proliferation; squamous cell carcinoma.