Subtle Alterations in DNA Methylation Patterns in Normal Cells in Response to Dietary Stilbenoids

Megan Beetch; Katarzyna Lubecka; Heather Kristofzski; Matthew Suderman; Barbara Stefanska

doi:10.1002/mnfr.201800193

Subtle Alterations in DNA Methylation Patterns in Normal Cells in Response to Dietary Stilbenoids

Mol Nutr Food Res. 2018 Jul;62(14):e1800193. doi: 10.1002/mnfr.201800193. Epub 2018 Jun 26.

Authors

Megan Beetch¹, Katarzyna Lubecka², Heather Kristofzski³, Matthew Suderman^{4

5}, Barbara Stefanska¹

Affiliations

¹ Food, Nutrition and Health Program, Faculty of Land and Food Systems, the University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.
² Department of Biomedical Chemistry, Medical University of Lodz, Lodz, 92-215, Poland.
³ Department of Nutrition Science, Purdue University, West Lafayette, IN, 47907, USA.
⁴ School of Social and Community Medicine, University of Bristol, Bristol, BS8 2BN, UK.
⁵ MRC Integrative Epidemiology Unit, University of Bristol, Bristol, BS8 2BN, UK.

PMID: 29797699
DOI: 10.1002/mnfr.201800193

Abstract

Scope: Searching for correlations between dietary polyphenols and risk of chronic diseases has been a challenge due to the lack of quantitative evaluation methods of long-term exposure. We previously observed substantial DNA methylation changes in human cancer cells upon treatment with polyphenols of the stilbenoid class. When induced in normal cells, such molecular changes may persist and reflect chronic exposure.

Methods and results: Illumina 450K microarray is used to delineate a genome wide DNA methylation landscape in MCF10A human immortalized mammary epithelial cells exposed to resveratrol (RSV) at noncytotoxic 15 μM dose for 9 days. Subtle alterations are observed suggesting remodeling of DNA methylation patterns rather than switch on/off changes. Using pyrosequencing, DNA methylation is quantitatively measured at eight CpG sites located within KCNJ4, RNF169, BCHE, DAOA, HOXA9, RUNX3, KRTAP2-1, and TAGAP, upon exposure to RSV or pterostilbene and shows similar differences induced by both stilbenoids. Two of the probes, Runx3 and Kcnj4, are successfully verified in whole blood DNA from healthy rats on diets supplemented with stilbenoids.

Conclusions: The study provides strong support for testing the utility of polyphenol-mediated changes in DNA methylation as quantitative measures of long-term dietary exposures in nutritional epidemiology and clinical trials.

Keywords: DNA methylation; diet; healthy cells; stilbenoids.

Abstract

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