Bioaugmentation potential of a newly isolated strain Sphingomonas sp. NJUST37 for the treatment of wastewater containing highly toxic and recalcitrant tricyclazole

Haobo Wu; Jinyou Shen; Xinbai Jiang; Xiaodong Liu; Xiuyun Sun; Jiansheng Li; Weiqing Han; Yang Mu; Lianjun Wang

doi:10.1016/j.biortech.2018.05.071

Bioaugmentation potential of a newly isolated strain Sphingomonas sp. NJUST37 for the treatment of wastewater containing highly toxic and recalcitrant tricyclazole

Bioresour Technol. 2018 Sep:264:98-105. doi: 10.1016/j.biortech.2018.05.071. Epub 2018 May 19.

Authors

Haobo Wu¹, Jinyou Shen², Xinbai Jiang¹, Xiaodong Liu¹, Xiuyun Sun¹, Jiansheng Li¹, Weiqing Han¹, Yang Mu³, Lianjun Wang¹

Affiliations

¹ Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
² Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: shenjinyou@mail.njust.edu.cn.
³ CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.

PMID: 29793119
DOI: 10.1016/j.biortech.2018.05.071

Abstract

In order to develop an effective bioaugmentation strategy for the removal of highly toxic and recalcitrant tricyclazole from wastewater, a tricyclazole degrading strain was firstly successfully isolated and identified as Sphingomonas sp. NJUST37. In batch reactors, 100 mg L^-1 tricyclazole could be completely removed within 102 h, which was accompanied by significant biomass increase, TOC and COD removal, as well as toxicity reduction. Chromatography analysis and density functional theory simulation indicated that monooxygenation occurred firstly, followed by triazole ring cleavage, decyanation reaction, hydration reaction, deamination, dihydroxylation and final mineralization reaction. Tricyclazole biodegradation condition by NJUST37 was optimized in terms of temperature, pH, tricyclazole concentration and additional carbon and nitrogen sources. After the inoculation of NJUST37 into a pilot-scale powdered activated carbon treatment tank treating real fungicide wastewater, tricyclazole removal efficiency increased to higher than 90%, demonstrating the great potential of NJUST37 for bioaugmentation particularly on tricyclazole biodegradation in practice.

Keywords: Bioaugmentation; Biodegradation; Metabolic pathway; Sphingomonas sp.; Tricyclazole.

MeSH terms

Biodegradation, Environmental
Sphingomonas*
Thiazoles / isolation & purification*
Wastewater*
Water Pollutants, Chemical / isolation & purification*

Substances

Thiazoles
Waste Water
Water Pollutants, Chemical
tricyclazole