Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells

Methods Mol Biol. 2019:2045:259-269. doi: 10.1007/7651_2018_164.

Abstract

Primordial germ cells (PGCs), the precursors of gametes, are the only cells capable of acquiring totipotency upon fertilization, but the molecular mechanisms regulating germ cell characteristics have not been fully elucidated. Although intracellular metabolic status and regulation are responsible for the control of cell function and differentiation, little is known about the metabolic features of PGCs. Here, we describe use of an integrated metabolomic, proteomic, and energy metabolic analysis method to comprehensively elucidate the metabolic characteristics of PGCs using mass spectrometry.

Keywords: Glycolysis; Metabolome; Oxidative phosphorylation; Primordial germ cells; Proteome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Liquid
  • Embryonic Germ Cells / cytology
  • Embryonic Germ Cells / drug effects
  • Embryonic Germ Cells / metabolism*
  • Female
  • Flow Cytometry
  • Green Fluorescent Proteins / metabolism
  • Metabolome / drug effects
  • Metabolome / physiology*
  • Metabolomics / methods*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Mouse Embryonic Stem Cells / cytology
  • Mouse Embryonic Stem Cells / drug effects
  • Mouse Embryonic Stem Cells / metabolism
  • Octamer Transcription Factor-3 / genetics
  • Octamer Transcription Factor-3 / metabolism
  • Oxidative Phosphorylation / drug effects
  • Oxygen Consumption / drug effects
  • Oxygen Consumption / physiology
  • Proteome / drug effects
  • Proteome / metabolism*
  • Proteomics / methods*
  • Tandem Mass Spectrometry

Substances

  • Octamer Transcription Factor-3
  • Pou5f1 protein, mouse
  • Proteome
  • Green Fluorescent Proteins