Bombesin functionalized 64Cu-copper sulfide nanoparticles for targeted imaging of orthotopic prostate cancer

Huawei Cai; Fang Xie; Aditi Mulgaonkar; Lihong Chen; Xiankai Sun; Jer-Tsong Hsieh; Fangyu Peng; Rong Tian; Lin Li; Changqiang Wu; Hua Ai

doi:10.2217/nnm-2018-0062

Bombesin functionalized ⁶⁴Cu-copper sulfide nanoparticles for targeted imaging of orthotopic prostate cancer

Nanomedicine (Lond). 2018 Jul 1;13(14):1695-1705. doi: 10.2217/nnm-2018-0062. Epub 2018 May 22.

Authors

Huawei Cai^{1

2}, Fang Xie^{2

3

4

5}, Aditi Mulgaonkar², Lihong Chen⁶, Xiankai Sun^{2

4}, Jer-Tsong Hsieh⁷, Fangyu Peng^{2

3

4}, Rong Tian¹, Lin Li¹, Changqiang Wu^{8

9}, Hua Ai⁹

Affiliations

¹ Department of Nuclear Medicine, Laboratory of Clinical Nuclear Medicine, West China Hospital, Sichuan University, Chengdu, 610041, PR China.
² Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
³ Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
⁴ Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
⁵ PET Center, Huashan Hospital, Fudan University, 200040, Shanghai, PR China.
⁶ Department of Biochemistry & Molecular Biology, West China School of Basic Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, PR China.
⁷ Department of Urology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
⁸ Sichuan Key Laboratory of Medical Imaging & School of Medical Imaging, North Sichuan Medical College, Nanchong, 637000, PR China.
⁹ National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, Sichuan, PR China.

PMID: 29786467
DOI: 10.2217/nnm-2018-0062

Abstract

Aim: To synthesize and evaluate the imaging potential of Bom-PEG-[⁶⁴Cu]CuS nanoparticles (NPs) in orothotopic prostate tumor. Materials & methods: [⁶⁴Cu]CuS NPs were synthesized in aqueous solution by ⁶⁴CuCl₂ and Na₂S reaction. Then PEG linker with or without bombesin peptide were conjugated to the surface of [⁶⁴Cu]CuS NPs to produce Bom-PEG-[⁶⁴Cu]CuS and PEG-[⁶⁴Cu]CuS NPs. These two kinds of NPs were used for testing specific uptake in prostate cancer cells in vitro and imaging of orthotopic prostate tumor in vivo. Results: Bom-PEG-[⁶⁴Cu]CuS and PEG-[⁶⁴Cu]CuS NPs were successfully synthesized with core diameter of approximately 5 nm. Radioactive cellular uptake revealed that Bom-PEG-[⁶⁴Cu]CuS was able to specifically bind to prostate cancer cells, and the microPET-CT imaging indicated clear visualization of orthotopic prostate tumors. Conclusion: Radiolabeled Bom-PEG-[⁶⁴Cu]CuS NPs have potential as an ideal agent for orthotopic prostate tumor imaging by microPET-CT.

Keywords: 64Cu-copper sulfide nanoparticles; bombesin; micro-PET-CT; prostate cancer.