Label-free liquid crystal biosensor for cecropin B detection

Jiao Zhang; Xiuxia Su; Dong Yang; Chonglin Luan

doi:10.1016/j.talanta.2018.04.004

Label-free liquid crystal biosensor for cecropin B detection

Talanta. 2018 Aug 15:186:60-64. doi: 10.1016/j.talanta.2018.04.004. Epub 2018 Apr 4.

Authors

Jiao Zhang¹, Xiuxia Su², Dong Yang¹, Chonglin Luan³

Affiliations

¹ College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
² College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China. Electronic address: suxiuxia@sust.edu.cn.
³ School of Applied Chemistry and Biotechnology, Shenzhen Polytechnic, Shenzhen 518088, China. Electronic address: luanchonglin@szpt.edu.cn.

PMID: 29784409
DOI: 10.1016/j.talanta.2018.04.004

Abstract

A label-free liquid crystal (LC) biosensor based on orientation changes of LC molecules was reported for the detection of cecropin B. The homeotropic-to-tilted alignment transition of LC molecules, induced by the specific binding event between cecropin B and anti-cecropin B antibody immobilized via glutaraldehyde, could result in obvious change of the optical appearance from a dark to a bright response and as a result, the detection limit of cecropin B was as low as 50 ng/mL. The average gray-scale intensities (GIs) of optical appearances were calculated to quantitatively analyse cecropin B concentrations. This study offers a simple, highly sensitive and specific, lable-free method for cecropin B detection.

Keywords: Anti-cecropin B antibody; Biosensor; Cecropin B; Liquid crystal.

MeSH terms

Antibodies / chemistry
Antibodies / immunology
Antigen-Antibody Reactions
Biosensing Techniques*
Insect Proteins / analysis*
Insect Proteins / immunology
Liquid Crystals / chemistry*
Microscopy, Atomic Force

Substances

Antibodies
Insect Proteins
cecropin B protein, Insecta