Alpha fetoprotein assessment by using a nano optical sensor thin film binuclear Pt-2-aminobenzimidazole-Bipyridine for early diagnosis of liver cancer

Abstract

A simple, precise and sensitive method in which, a nano optical sensor binuclear Pt-2-aminobenzimidazole-bipyridine, Pt(abi)(bpy) complex doped in sol gel is used for the early diagnosis of liver cancer. The idea depends on the assessment of the concentration of alpha fetoprotein (AFP) in the serum samples of different liver patients. The nano binuclear Pt(abi)(bpy) has strong emission spectrum upon excitation at 380 nm in distilled water. The assessment of alpha fetoprotein (AFP) depends on the quenching of the emission spectrum of the optical sensor at 610 nm in water by the alpha fetoprotein (AFP). The calibration plot was achieved over the concentration 5.0 - 350 U L^-1 with a correlation coefficient of 0.997 and a detection limit of 3.0 U L^-1. The method was used satisfactorily for the diagnosis of liver cancer in a number of serum samples collected from various patients and health state; healthy (≤ 30 U L^-1), Virus B (42.2-69.5 U L^-1), Virus C (75.7-98.4 U L^-1), Cirrhosis (112-147 U L^-1) and HCC (185.2-349.6 U L^-1). Furthermore, the assessment of the alpha-fetoprotein by the proposed method increases its sensitivity (92.88%) and specificity (91.41%) for early diagnosis of HCC.

Keywords: Alpha fetoprotein (AFP); Liver cancer; Luminescence; Nano binuclear Pt(abi)(bpy); Optical sensor.