C-lysozyme contributes to antiviral immunity in Bombyx mori against nucleopolyhedrovirus infection

Abstract

Lysozymes is a ubiquitous immune effector that is widely distributed in both vertebrates and invertebrates. Previous reports have shown that lysozymes significantly inhibit viral infections in vertebrates. However, the antiviral effects of lysozymes in invertebrates remain unclear. Here, we investigated the role of lysozymes in Bombyx mori (B. mori) response to viral infection by overexpressing B. mori C-lysozyme (BmC-LZM) in larvae and cells. We found that BmC-LZM was up-regulated in cells in response to viral infection. Indeed, the overexpressing of BmC-LZM significantly inhibited viral replication in cells during late-stage infection. However, this effect was reversed by BmC-LZM mRNA. BmC-LZM was successfully overexpressed in B. mori strain 871 using Baculovirus Expression Vector System (BEVS). This overexpression markedly reduced viral proliferation and increased larval survival percentage. Thus, BmC-LZM inhibited viral replication both in vivo and in vitro, indicating that BmC-LZM is involved in the insect immune response to viral infection. Our results provide a basis for further applications of lysozymes.

Keywords: Antivirus; BmNPV; Bombyx mori; Insect; Lysozyme.