Diagnostic Molecular Microbiology: A 2018 Snapshot

Marilynn Ransom Fairfax; Martin H Bluth; Hossein Salimnia

doi:10.1016/j.cll.2018.02.004

Diagnostic Molecular Microbiology: A 2018 Snapshot

Clin Lab Med. 2018 Jun;38(2):253-276. doi: 10.1016/j.cll.2018.02.004.

Authors

Marilynn Ransom Fairfax¹, Martin H Bluth², Hossein Salimnia³

Affiliations

¹ Department of Pathology, Wayne State University School of Medicine, 540 East Canfield Street, Detroit, MI 48201, USA; Clinical Microbiology Laboratories, DMC University Laboratories, 4201 St. Antoine Street, Detroit, MI 48201, USA. Electronic address: mfairfax@dmc.org.
² Department of Pathology, Wayne State University School of Medicine, 540 East Canfield Street, Detroit, MI 48201, USA; Pathology Laboratories, Michigan Surgical Hospital, 21230 Dequindre Road, Warren, MI 48091, USA.
³ Department of Pathology, Wayne State University School of Medicine, 540 East Canfield Street, Detroit, MI 48201, USA; Clinical Microbiology Laboratories, DMC University Laboratories, 4201 St. Antoine Street, Detroit, MI 48201, USA.

Abstract

Molecular biological techniques have evolved expeditiously and in turn have been applied to the detection of infectious disease. Maturation of these technologies and their coupling with related technological advancement in fluorescence, electronics, digitization, nanodynamics, and sensors among others have afforded clinical medicine additional tools toward expedient identification of infectious organisms at concentrations and sensitivities previously unattainable. These advancements have been adapted in select settings toward addressing clinical demands for more timely and effective patient management.

Keywords: MALDI-TOF; Molecular microbiology; Next gen sequencing; Nuclear magnetic resonance; PCR; Probe tests; Rapid molecular diagnosis of infections; Time lapsed imaging.

Publication types

Review

MeSH terms

High-Throughput Nucleotide Sequencing
Humans
Magnetic Resonance Spectroscopy
Microbiological Techniques*
Molecular Diagnostic Techniques*
Pathology, Molecular*
Polymerase Chain Reaction
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization