Complete genome sequence of Achromobacter spanius type strain DSM 23806T, a pathogen isolated from human blood

Gengmi Li; Li Yang; Tao Zhang; Xiaojiao Guo; Jian Qin; Yingjiang Cao; Qianhua Yang; Shumei You; Guoliang Yuan; Xianqi Wan; Jing Luo; Zhaoxiang Li; Lei Gao; Ying Liu; Kaifeng Jiang; Jiakui Zheng

doi:10.1016/j.jgar.2018.05.003

Complete genome sequence of Achromobacter spanius type strain DSM 23806^T, a pathogen isolated from human blood

J Glob Antimicrob Resist. 2018 Sep:14:1-3. doi: 10.1016/j.jgar.2018.05.003. Epub 2018 Jun 8.

Authors

Gengmi Li¹, Li Yang¹, Tao Zhang¹, Xiaojiao Guo¹, Jian Qin¹, Yingjiang Cao¹, Qianhua Yang¹, Shumei You², Guoliang Yuan³, Xianqi Wan³, Jing Luo², Zhaoxiang Li², Lei Gao³, Ying Liu², Kaifeng Jiang⁴, Jiakui Zheng⁵

Affiliations

¹ Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, PR China; Luzhou Branch of National Rice Improvement Center, Luzhou 646100, PR China.
² Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, PR China.
³ Luzhou Branch of National Rice Improvement Center, Luzhou 646100, PR China.
⁴ Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, PR China. Electronic address: jiangkf67@126.com.
⁵ Luzhou Branch of National Rice Improvement Center, Luzhou 646100, PR China. Electronic address: zhen6102@126.com.

PMID: 29775788
DOI: 10.1016/j.jgar.2018.05.003

Abstract

Objectives: Achromobacter spanius is a newly described, non-fermenting, Gram-negative, coccoid pathogen isolated from human blood. Whole-genome sequencing of the A. spanius type strain was performed to investigate the mechanism of pathogenesis of this strain at a genomic level.

Methods: The complete genome of A. spanius type strain DSM 23806^T was sequenced using single-molecule real-time (SMRT) DNA sequencing.

Results: The complete genome of DSM 23806^T consists of one circular DNA chromosome of 6425783bp with a G+C content of 64.26%. The entire genome contains 5804 predicted coding sequences (CDS) and 55 tRNAs. Genomic island (GI) analysis showed that this strain encodes several important pathogenesis- and resistance-related genes.

Conclusions: These results strongly suggest that GIs provide some fitness advantages in A. spanius type strain DSM 23806^T. This report provides an extensive understanding of A. spanius at a genomic level as well as an understanding of the evolution of A. spanius.

Keywords: Achromobacter spanius; Genomic island; PacBio.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Achromobacter / genetics*
Achromobacter / isolation & purification
Achromobacter / pathogenicity*
China
DNA, Bacterial / genetics
Genome, Bacterial*
Genomics
Gram-Negative Bacterial Infections / blood
Humans
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Whole Genome Sequencing

Substances

DNA, Bacterial
RNA, Ribosomal, 16S