[Expression and clinical significance of Testin in nasopharyngeal carcinoma]

Abstract

Objective:Our purpose was to investigate the expression of Testin gene,and its possible relationship with the clinicopathological features of human nasopharyngeal carcinoma.Method:The expression of Testin in nasopharyngeal carcinoma tissues were detected by immunohistochemistry methods,semi-quantitative reverse transcriptase polymerase chain reaction and Western blot.The correlations of Testin to clinicopathologic features of nasopharyngeal carcinoma were analyzed.Result:The mRNA level of Testin was down-regulated in human nasopharyngeal carcinoma.The positive rate of Testin protein was significantly lower in human nasopharyngeal carcinoma tissues than that in nomal tissues;The protein level of Testin was down-regulated in cancers as compared with corresponding normal tissues.Testin expression was positively correlated with the differentiation of nasopharyngeal carcinoma.Meanwhile,differences in gender and age were not significance(P>0.05 respectively) .There was a significant correlation between invasion,distant metastasis and differentiation degree and Testin expression(P<0.05 respectively).Conclusion:The decreased expression of Testin gene may play an importmant role in the development of esophageal squamous cancer.Thus Testin gene might be a novel candidate of tumor-suppressor.It may be an objective marker for prognostic factor and malignant level for nasopharyngeal carcinoma.

Keywords: Testin gene; Western blots; immunohistochemistry; nasopharyngeal neoplasms; reverse transcriptase polymerase chain reaction.