Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems

Mugui Wang; Yanfei Mao; Yuming Lu; Zhidan Wang; Xiaoping Tao; Jian-Kang Zhu

doi:10.1111/jipb.12667

Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems

J Integr Plant Biol. 2018 Aug;60(8):626-631. doi: 10.1111/jipb.12667. Epub 2018 Jul 7.

Authors

Mugui Wang¹, Yanfei Mao¹, Yuming Lu¹, Zhidan Wang¹, Xiaoping Tao¹, Jian-Kang Zhu^{1

2}

Affiliations

¹ Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China.
² Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA.

PMID: 29762900
DOI: 10.1111/jipb.12667

Abstract

We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co-expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing.

Publication types

Letter

MeSH terms

CRISPR-Cas Systems / genetics*
Clustered Regularly Interspaced Short Palindromic Repeats / genetics
Gene Editing*
Oryza / genetics*
Plant Proteins / genetics
Plant Proteins / metabolism

Substances

Plant Proteins