Mass Spectrometry Imaging (MSI) for Plant Metabolomics

Berin A Boughton; Dinaiz Thinagaran

doi:10.1007/978-1-4939-7819-9_17

Mass Spectrometry Imaging (MSI) for Plant Metabolomics

Methods Mol Biol. 2018:1778:241-252. doi: 10.1007/978-1-4939-7819-9_17.

Authors

Berin A Boughton¹, Dinaiz Thinagaran²

Affiliations

¹ Metabolomics Australia, School of BioSciences, The University of Melbourne, Parkville, VIC, Australia. baboug@unimelb.edu.au.
² Metabolomics Australia, School of BioSciences, The University of Melbourne, Parkville, VIC, Australia.

PMID: 29761443
DOI: 10.1007/978-1-4939-7819-9_17

Abstract

Mass spectrometry imaging (MSI) is a developing technique to measure the spatiotemporal distribution of many biomolecules in tissues. Over the preceding decade MSI has been adopted by plant biologists and applied in a broad range of areas including: primary metabolism, natural products, plant defense, plant responses to abiotic and biotic stress, plant lipids, and the developing field of spatial metabolomics. This methods chapter covers preparation of plant tissues for matrix-assisted laser desorption ionization (MALDI)-MSI, including sample embedding and freezing, sectioning, mounting, and matrix deposition using both sublimation and spray deposition prior to MSI analysis.

Keywords: Frozen section; MALDI; MSI analysis; Mass spectrometry imaging; Matrix; Matrix-assisted laser desorption ionization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Mass Spectrometry / methods*
Metabolomics / methods*
Plants / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization