Macrophage activation is a process that is highly relevant in both infectious and chronic diseases and so the study of macrophage activation is of high interest to investigators in many fields of biomedical research. Bone marrow-derived macrophages are a popular choice for studying macrophage activation in vitro, as these cells are relatively hardy and many macrophages can be produced from just one mouse. Here we describe the activation of mouse macrophages in vitro, including special considerations for cell culture, detachment, kinetics, and activation states.
Keywords: BMDMs; Classically activated macrophages; Interferon gamma; Lipopolysaccharide; M1; Macrophage activation; Macrophages.