Demonstrating the viability of halolipase production at a mechanically stirred tank biological reactor

Sergio Colangiuli; Ana Rodríguez; María Ángeles Sanromán; Francisco J Deive

doi:10.1016/j.biortech.2018.05.017

Demonstrating the viability of halolipase production at a mechanically stirred tank biological reactor

Bioresour Technol. 2018 Sep:263:334-339. doi: 10.1016/j.biortech.2018.05.017. Epub 2018 May 4.

Authors

Sergio Colangiuli¹, Ana Rodríguez¹, María Ángeles Sanromán¹, Francisco J Deive²

Affiliations

¹ Department of Chemical Engineering, University of Vigo, 36310 Vigo, Spain.
² Department of Chemical Engineering, University of Vigo, 36310 Vigo, Spain. Electronic address: deive@uvigo.es.

PMID: 29758483
DOI: 10.1016/j.biortech.2018.05.017

Abstract

The definition of halophiles as "the coming stars of industrial biotechnology" in a recent review demands new research efforts for their efficient production at bioreactor scale. In this sense, the scarcity of information about halolipases production has furthered the research on the viability of Halomonas sp. LM1C culture in a mechanically stirred bioreactor. The operating conditions have been optimized by means of a Central Composite Face-Centered (CCFC) design. The operation at low aerations (0.25 vvm) and moderate agitation rates (583 rpm) led to activity levels near 8000 U/L, which clearly surpasses the typical values detected for other extremophilic enzymes. The process at optimum conditions has been kinetically characterized and the oxygen volumetric mass transfer coefficient (K_La) has been determined.

Keywords: Extremophile; Halomonas; Halophile; Lipase production; Response surface methodology.

MeSH terms

Bioreactors*
Biotechnology
Halomonas*
Oxygen

Substances

Oxygen