Ionic liquids (ILs), which are alternatives to traditional organic solvents, have the potential to enter soil and cause negative effects on the soil micro-environment, especially soil organisms. The objective of this study was to determine the "alkyl chain effect" and "cut-off effect" mechanisms underlying the toxicity of ILs. The assessment for subchronic toxicity toward earthworms (Eisenia fetida) by five common imidazole nitrate ILs ([Cnmim]NO3 (n = 4, 6, 8, 10, and 12)) was conducted on day 28 after exposure to five concentrations (0, 5, 10, 20, and 40 mg kg-1) of ILs. Earthworms showed oxidative stress and oxidative damage, and both "alkyl chain effect" and "cut-off effect" (occurred in C10) were observed. In addition, the toxicity of ILs increased with the increase in concentration. Analysis of imidazolium ILs in artificial soil at the end of the experiment indicated that these selected ILs remained relatively stable, with a rate of change of less than 7.39%. The present study provides theoretical support for decisions regarding IL use and helps to establish a friendly IL structure database.
Keywords: Earthworms; Oxidative damage; Oxidative stress; [C(n)mim]NO(3).
Copyright © 2018 Elsevier Ltd. All rights reserved.