Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film

Shamima Akhter; Wan Jefrey Basirun; Yatimah Alias; Mohd Rafie Johan; Samira Bagheri; Md Shalauddin; Magaji Ladan; Nadzirah Sofia Anuar

doi:10.1016/j.ab.2018.05.004

Enhanced amperometric detection of paracetamol by immobilized cobalt ion on functionalized MWCNTs - Chitosan thin film

Anal Biochem. 2018 Jun 15:551:29-36. doi: 10.1016/j.ab.2018.05.004. Epub 2018 May 16.

Authors

Shamima Akhter¹, Wan Jefrey Basirun², Yatimah Alias³, Mohd Rafie Johan⁴, Samira Bagheri⁵, Md Shalauddin⁴, Magaji Ladan⁶, Nadzirah Sofia Anuar⁷

Affiliations

¹ Department of Chemistry, Faculty of Science, University Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: shamimaakhter053@yahoo.com.
² Department of Chemistry, Faculty of Science, University Malaya, 50603, Kuala Lumpur, Malaysia; Nanotechnology and Catalysis Research Center (NANOCAT), University Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: jeff@um.edu.my.
³ Department of Chemistry, Faculty of Science, University Malaya, 50603, Kuala Lumpur, Malaysia; University Malaya Centre for Ionic Liquids (UMCiL), University Malaya, 50603, Kuala Lumpur, Malaysia.
⁴ Nanotechnology and Catalysis Research Center (NANOCAT), University Malaya, 50603, Kuala Lumpur, Malaysia.
⁵ Department of Mechanical Engineering, Tufts University, 200 College Ave, Medford, MA, 02155, USA.
⁶ Department of Chemistry, Bayero University, Kano, Nigeria.
⁷ Department of Chemistry, Faculty of Science, University Malaya, 50603, Kuala Lumpur, Malaysia.

PMID: 29753720
DOI: 10.1016/j.ab.2018.05.004

Abstract

In the present study, a nanocomposite of f-MWCNTs-chitosan-Co was prepared by the immobilization of Co(II) on f-MWCNTs-chitosan by a self-assembly method and used for the quantitative determination of paracetamol (PR). The composite was characterized by field emission scanning electron microscopy (FESEM) and energy dispersive x-ray analysis (EDX). The electroactivity of cobalt immobilized on f-MWCNTs-chitosan was assessed during the electro-oxidation of paracetamol. The prepared GCE modified f-MWCNTs/CTS-Co showed strong electrocatalytic activity towards the oxidation of PR. The electrochemical performances were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). Under favorable experimental conditions, differential pulse voltammetry showed a linear dynamic range between 0.1 and 400 μmol L^-1 with a detection limit of 0.01 μmol L^-1 for the PR solution. The fabricated sensor exhibited significant selectivity towards PR detection. The fabricated sensor was successfully applied for the determination of PR in commercial tablets and human serum sample.

Keywords: Chitosan; Cobalt; Electrochemical sensor; Paracetamol; f-Multi-wall carbon nanotube.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Acetaminophen / analysis*
Acetaminophen / blood
Acetaminophen / toxicity
Analgesics, Non-Narcotic / analysis
Analgesics, Non-Narcotic / blood
Antipyretics / analysis
Antipyretics / blood
Biosensing Techniques / methods
Chitosan / chemistry
Cobalt / chemistry
Dielectric Spectroscopy
Electrochemical Techniques / methods*
Humans
Hydrogen-Ion Concentration
Metal Nanoparticles / chemistry
Metal Nanoparticles / ultrastructure
Microscopy, Electron, Scanning
Nanocomposites / chemistry
Nanocomposites / ultrastructure
Nanotubes, Carbon / chemistry
Nanotubes, Carbon / ultrastructure
Spectroscopy, Fourier Transform Infrared
Tablets / chemistry

Substances

Analgesics, Non-Narcotic
Antipyretics
Nanotubes, Carbon
Tablets
Acetaminophen
Cobalt
Chitosan