Species distribution, virulence factors, and antimicrobial resistance of Acinetobacter spp. isolates from dogs and cats: a preliminary study

Yui Kimura; Kazuki Harada; Takae Shimizu; Tomomi Sato; Akari Kajino; Masaru Usui; Yutaka Tamura; Yuzo Tsuyuki; Tadashi Miyamoto; Asami Ohki; Masahisa Watarai

doi:10.1111/1348-0421.12601

Species distribution, virulence factors, and antimicrobial resistance of Acinetobacter spp. isolates from dogs and cats: a preliminary study

Microbiol Immunol. 2018 May 12. doi: 10.1111/1348-0421.12601. Online ahead of print.

Authors

Yui Kimura^{1

2}, Kazuki Harada^{1

3}, Takae Shimizu^{1

3}, Tomomi Sato⁴, Akari Kajino⁴, Masaru Usui⁴, Yutaka Tamura⁴, Yuzo Tsuyuki⁵, Tadashi Miyamoto², Asami Ohki⁶, Masahisa Watarai¹

Affiliations

¹ The United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
² Miyamoto Animal Hospital, 2265-8 Kurokawa, Yamaguchi 753-0851, Japan.
³ Department of Veterinary Internal Medicine, Tottori University, Minami 4-101, Koyama, Tottori 680-8553, Japan.
⁴ Laboratory of Food Microbiology and Food Safety, Rakuno Gakuen University, 582 Midori, Bunkyodai, Ebetsu, Hokkaido 069-8501, Japan.
⁵ Sanritsu Zelkova Veterinary Laboratory, 2-5-8 Kuji, Takatsu, Kawasaki, Kanagawa 213-0032, Japan.
⁶ Fujifilm Monoris Co., Ltd., 1-49-18 Nishikoigakubo, Kokubunji, Tokyo 185-0013, Japan.

PMID: 29752821
DOI: 10.1111/1348-0421.12601

Abstract

We investigated the prevalence of virulence factors and antimicrobial resistance among 67 Acinetobacter spp. isolates, consisting of 21 Acinetobacter baumannii and 46 non-baumannii Acinetobacter from companion animals. The PCR analysis showed that the most prevalent virulence gene was afa/draBC (29.9%), followed by papC (22.4%) and cvaC (20.9%). Antimicrobial susceptibility testing revealed that resistance to gentamicin (14.9%) and ciprofloxacin (11.9%) was relatively prevalent. Five gentamicin- and/or ciprofloxacin-resistant A. baumannii strains were assigned to ST25, ST149, ST164, ST203, and ST1198. All ciprofloxacin-resistant isolates harbored point mutations in gyrA and/or parC. This is the first preliminary monitoring of animal-origin Acinetobacter spp. in Japan.

Keywords: Acinetobacter spp; antimicrobial resistance; virulence factor.