Adsorption of lysozyme by alginate/graphene oxide composite beads with enhanced stability and mechanical property

Jiwei Li; Jianwei Ma; Shaojuan Chen; Yudong Huang; Jinmei He

doi:10.1016/j.msec.2018.03.023

Adsorption of lysozyme by alginate/graphene oxide composite beads with enhanced stability and mechanical property

Mater Sci Eng C Mater Biol Appl. 2018 Aug 1:89:25-32. doi: 10.1016/j.msec.2018.03.023. Epub 2018 Mar 27.

Authors

Jiwei Li¹, Jianwei Ma², Shaojuan Chen², Yudong Huang³, Jinmei He⁴

Affiliations

¹ College of Textiles and Clothing, Qingdao University,Qingdao, Shandong 266071, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.
² College of Textiles and Clothing, Qingdao University,Qingdao, Shandong 266071, China.
³ State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.
⁴ State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China. Electronic address: hejinmei@hit.edu.cn.

PMID: 29752096
DOI: 10.1016/j.msec.2018.03.023

Abstract

The large-scale applications of lysozyme in the pharmaceutical industry and food industry require more efficient and cost-effective techniques for its separation/purification. In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca²⁺ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions. Compared with pure Ca²⁺ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance. This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA. Batch adsorption experiments found that the lysozyme adsorption capacity of Ca-SA/GO can reach 278.28 mg g^-1 and it can be regenerated and reused at least 4 times. Moreover, in column adsorption, the Ca-SA/GO showed excellent dynamic adsorption property. With good stability, adsorption capacity, and regeneration ability, the Ca-SA/GO could be a promising adsorbent for lysozyme from aqueous solutions.

Keywords: Alginate; Graphene oxide; Lysozyme; Mechanical properties.

MeSH terms

Adsorption
Alginates / chemistry*
Calcium / chemistry
Compressive Strength
Glucuronic Acid / chemistry
Graphite / chemistry*
Hexuronic Acids / chemistry
Microscopy, Electron, Scanning
Muramidase / chemistry*
Oxides / chemistry
Particle Size
Spectroscopy, Fourier Transform Infrared
Thermogravimetry
X-Ray Diffraction

Substances

Alginates
Hexuronic Acids
Oxides
Graphite
Glucuronic Acid
Muramidase
Calcium