A strategy for untargeted screening of macrolides and metabolites in bass by liquid chromatography coupled to quadrupole orbitrap mass spectrometry

Wei Jia; Lin Shi; Xiaogang Chu; James Chang; Ying Chen; Feng Zhang

doi:10.1016/j.foodchem.2018.04.090

A strategy for untargeted screening of macrolides and metabolites in bass by liquid chromatography coupled to quadrupole orbitrap mass spectrometry

Food Chem. 2018 Oct 1:262:110-117. doi: 10.1016/j.foodchem.2018.04.090. Epub 2018 Apr 23.

Authors

Wei Jia¹, Lin Shi², Xiaogang Chu², James Chang³, Ying Chen⁴, Feng Zhang⁴

Affiliations

¹ School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China. Electronic address: foodjiawei@aliyun.com.
² School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
³ Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, CA 95134, United States.
⁴ Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.

PMID: 29751897
DOI: 10.1016/j.foodchem.2018.04.090

Abstract

An analytical method for the non-target screening of macrolides and metabolites in bass (Lateolabrax) was developed using an automated on-line extraction procedure followed by ultrahigh-performance liquid chromatography coupled to electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC Q-Orbitrap). The estimated performance characteristics were satisfied, complying with the requirements of the guidelines specified in European Commission Decision 2002/657/EC. The decision limit ranged from 0.12 μg kg^-1 to 3.61 μg kg^-1, and detection capability ranged between 0.20 μg kg^-1 and 6.02 μg kg^-1. Precision in terms of relative standard deviation (RSD) was under 14% for all compounds, and the extraction recoveries ranged from 81% to 107%. Finally, the method was applied to ten different commercially important bass species and confirmed the presence of ten macrolides and metabolites. Five non-target compounds of robenidine, lincomycin hydrochloride, thiacloprid, fenbendazole, and thiabendazole were elucidated in the untargeted screening.

Keywords: Macrolides; Metabolites; UHPLC/ESI Q-Orbitrap; Untargeted screening.

MeSH terms

Animals
Bass / metabolism*
Chromatography, High Pressure Liquid / methods*
Macrolides / analysis*
Spectrometry, Mass, Electrospray Ionization / methods*

Substances

Macrolides