High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei

G Girault; P Wattiau; M Saqib; B Martin; F Vorimore; H Singha; M Engelsma; H J Roest; S Spicic; R Grunow; N Vicari; S C J De Keersmaecker; N H C Roosens; M Fabbi; B N Tripathi; S Zientara; N Madani; K Laroucau

doi:10.1016/j.meegid.2018.05.004

High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei

Infect Genet Evol. 2018 Sep:63:1-4. doi: 10.1016/j.meegid.2018.05.004. Epub 2018 May 8.

Authors

G Girault¹, P Wattiau², M Saqib³, B Martin¹, F Vorimore¹, H Singha⁴, M Engelsma⁵, H J Roest⁵, S Spicic⁶, R Grunow⁷, N Vicari⁸, S C J De Keersmaecker⁹, N H C Roosens⁹, M Fabbi⁸, B N Tripathi⁴, S Zientara¹, N Madani¹, K Laroucau¹⁰

Affiliations

¹ Paris-Est University, ANSES, Laboratory for Animal Health, Bacterial Zoonosis Unit, European Union Reference Laboratory for Equine Diseases/Glanders, Maisons-Alfort, France.
² Department of Bacterial Diseases, CODA-CERVA (Veterinary and Agrochemical Research Centre), Brussels, Belgium.
³ University of agriculture, Faisalabad, Pakistan.
⁴ ICAR-National Research Centre on Equines, Hisar, India.
⁵ WageningenBioveterinary Research, Lelystad, The Netherlands.
⁶ Croatian Veterinary Institute, Department for Bacteriology and Parasitology, Laboratory for Bacterial Zoonoses and Molecular Diagnosis of Bacterial Diseases, Zagreb, Croatia.
⁷ Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany.
⁸ IstitutoZooprofilatticoSperimentaledellaLombardia e dell'EmiliaRomagna "Bruno Ubertini", Pavia, Italy.
⁹ Platform Biotechnology and Molecular Biology, Scientific Institute of Public Health, Brussels, Belgium.
¹⁰ Paris-Est University, ANSES, Laboratory for Animal Health, Bacterial Zoonosis Unit, European Union Reference Laboratory for Equine Diseases/Glanders, Maisons-Alfort, France. Electronic address: karine.laroucau@anses.fr.

PMID: 29751195
DOI: 10.1016/j.meegid.2018.05.004

Abstract

Burkholderia (B.) mallei is the causative agent of glanders. A previous work conducted on single-nucleotide polymorphisms (SNP) extracted from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically informative SNPs within the genome of B. mallei that subtype the species into 3 lineages and 12 branches/sub-branches/groups. The present results demonstrate that SNP-based genotyping represent an interesting approach for the molecular epidemiology analysis of B. mallei.

Keywords: Burkholderia mallei; Genotyping; HRM.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Burkholderia mallei / classification
Burkholderia mallei / genetics*
DNA, Bacterial / genetics*
Genotype*
Phylogeny
Polymerase Chain Reaction / methods*
Polymorphism, Single Nucleotide

Substances

DNA, Bacterial