Dendritic Ca2+ dynamics and multimodal processing in a cricket antennal interneuron

Abstract

The integration of stimuli of different modalities is fundamental to information processing within the nervous system. A descending interneuron in the cricket brain, with prominent dendrites in the deutocerebrum, receives input from three sensory modalities: touch of the antennal flagellum, strain of the antennal base, and visual stimulation. Using calcium imaging, we demonstrate that each modality drives a Ca²⁺ increase in a different dendritic region. Moreover, touch of the flagellum is represented in a topographic map along the neuron's dendrites. Using intracellular recording, we investigated the effects of Ca²⁺ on spike shape through the application of the Ca²⁺ channel antagonist Cd²⁺ and identified probable Ca²⁺-dependent K⁺ currents. NEW & NOTEWORTHY Different dendritic regions of the cricket brain neuron DBNi1-2 showed localized Ca²⁺ increases when three modalities of stimulation (touch of the flagellum, strain at antennal base, and visual input) were given. Touch stimulation induces localized Ca²⁺ increases according to a topographic map of the antenna. Ca²⁺ appears to activate K⁺ currents in DBNi1-2.

Keywords: Ca2+ imaging; dendritic processing; multimodal stimulation.