Investigation on hydrogen production from paper sludge without inoculation and its enhancement by Clostridium thermocellum

Qian An; Ji-Lian Wang; Yu-Tao Wang; Zhang-Lin Lin; Ming-Jun Zhu

doi:10.1016/j.biortech.2018.04.105

Investigation on hydrogen production from paper sludge without inoculation and its enhancement by Clostridium thermocellum

Bioresour Technol. 2018 Sep:263:120-127. doi: 10.1016/j.biortech.2018.04.105. Epub 2018 Apr 30.

Authors

Qian An¹, Ji-Lian Wang², Yu-Tao Wang², Zhang-Lin Lin³, Ming-Jun Zhu⁴

Affiliations

¹ State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, People's Republic of China; School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou 510006, People's Republic of China.
² College of Life and Geographic Sciences, Kashgar University, Kashgar 844000, People's Republic of China; The Key Laboratory of Ecology and Biological Resources in Yarkand Oasis at Colleges & Universities under the Department of Education of Xinjiang Uygur Autonomous Region, Kashgar University, Kashgar 844000, People's Republic of China.
³ State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, People's Republic of China; School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou 510006, People's Republic of China. Electronic address: zhanglinlin@scut.edu.cn.
⁴ State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, People's Republic of China; School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou 510006, People's Republic of China; College of Life and Geographic Sciences, Kashgar University, Kashgar 844000, People's Republic of China; The Key Laboratory of Ecology and Biological Resources in Yarkand Oasis at Colleges & Universities under the Department of Education of Xinjiang Uygur Autonomous Region, Kashgar University, Kashgar 844000, People's Republic of China. Electronic address: mjzhu@scut.edu.cn.

PMID: 29738974
DOI: 10.1016/j.biortech.2018.04.105

Abstract

The feasibility and performance of hydrogen production from paper sludge without inoculation was investigated under thermophilic conditions. The maximum hydrogen production reached 64.32 mM with 7.4% PS. The dynamic changes in bacterial community structures during hydrogen production were investigated by analyzing 16S rDNA gene sequences using high throughput sequencing technology. The results showed that microbial community was dominated by order Clostridiales and Thermoanaerobacterales. Genus Thermoanaerobacterium and Ruminiclostridium played a leading role in the fermentation process, which was responsible for the hydrolysis of PS and hydrogen production. Effect of inoculation with Clostridium thermocellum on hydrogen production from PS was also studied. The results showed that C. thermocellum supplement significantly increased hydrogen yield and holocellulose degradation rate by 96.80% and 32.95%, respectively. In addition, inoculation of C. thermocellum enhanced VFA generation and shortened the lag phase of hydrogen production. The present study lays the foundation on the valorization of waste lignocellulose.

Keywords: Bacterial community; Clostridium thermocellum; Enhancement; Hydrogen production; Paper sludge.

MeSH terms

Cellulose
Clostridium thermocellum*
Fermentation
Hydrogen*
Lignin
Sewage
Thermoanaerobacterium

Substances

Sewage
Hydrogen
Cellulose
Lignin