Paper-Based Microfluidic Platforms for Understanding the Role of Exosomes in the Pathogenesis of Major Blindness-Threatening Diseases

Min-Yen Hsu; Chun-Chih Chiu; Juan-Yuan Wang; Chin-Te Huang; Yu-Fang Huang; Jyh-Cheng Liou; Chihchen Chen; Hung-Chi Chen; Chao-Min Cheng

doi:10.3390/nano8050310

Paper-Based Microfluidic Platforms for Understanding the Role of Exosomes in the Pathogenesis of Major Blindness-Threatening Diseases

Nanomaterials (Basel). 2018 May 8;8(5):310. doi: 10.3390/nano8050310.

Authors

Min-Yen Hsu^{1

2}, Chun-Chih Chiu³, Juan-Yuan Wang⁴, Chin-Te Huang⁵, Yu-Fang Huang⁶, Jyh-Cheng Liou^{7

8

9

10}, Chihchen Chen¹¹, Hung-Chi Chen¹², Chao-Min Cheng¹³

Affiliations

¹ Department of Ophthalmology, Chung Shan Medical University Hospital, Taichung 402, Taiwan. my.scott.hsu@gmail.com.
² School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan. my.scott.hsu@gmail.com.
³ Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan. hank011028@gmail.com.
⁴ Department of Ophthalmology, Taichung Veterans General Hospital, Taichung 407, Taiwan. cywang0926@yahoo.com.tw.
⁵ Institute of Medical Sciences, Tzu-Chi University, Hualien 970, Taiwan. chintehuang@hotmail.com.
⁶ Department of Ophthalmology, Taichung Veterans General Hospital, Taichung 407, Taiwan. i5496604@gmail.com.
⁷ Department of Ophthalmology, Chung Shan Medical University Hospital, Taichung 402, Taiwan. liou.outcast@gmail.com.
⁸ School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan. liou.outcast@gmail.com.
⁹ Department of Optometry, Chung Shan Medical University, Taichung 407, Taiwan. liou.outcast@gmail.com.
¹⁰ Institute of Medicine, Chung Shan Medical University, Taichung 407, Taiwan. liou.outcast@gmail.com.
¹¹ Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 300, Taiwan. chihchen23@gmail.com.
¹² Department of Ophthalmology and Department of Medicine, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Linkou 244 and Taoyuan 333, Taiwan. mr3756@cgmh.org.tw.
¹³ Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan. chaomin@mx.nthu.edu.tw.

Abstract

Emerging roles of exosomes in the pathogenesis of major blindness-threatening diseases, such as age-related macular degeneration, glaucoma, and corneal dystrophy, were discovered by aqueous humor analysis. A new diagnostic method using cellulose-based devices and microfluidic chip techniques for the isolation of exosomes from aqueous humor is less cumbersome and saves time. This method will enable more investigations for aqueous humor analysis in the future.

Keywords: Glaucoma; age-related macular degeneration; exosomes; lab on a chip; microfluidics.