Microfluidics-Enabled Multimaterial Maskless Stereolithographic Bioprinting

Amir K Miri; Daniel Nieto; Luis Iglesias; Hossein Goodarzi Hosseinabadi; Sushila Maharjan; Guillermo U Ruiz-Esparza; Parastoo Khoshakhlagh; Amir Manbachi; Mehmet Remzi Dokmeci; Shaochen Chen; Su Ryon Shin; Yu Shrike Zhang; Ali Khademhosseini

doi:10.1002/adma.201800242

Microfluidics-Enabled Multimaterial Maskless Stereolithographic Bioprinting

Adv Mater. 2018 Jul;30(27):e1800242. doi: 10.1002/adma.201800242. Epub 2018 May 7.

Authors

Amir K Miri^{1

2}, Daniel Nieto^{1

2

3}, Luis Iglesias^{1

2}, Hossein Goodarzi Hosseinabadi^{1

2

4}, Sushila Maharjan^{1

2}, Guillermo U Ruiz-Esparza^{1

2}, Parastoo Khoshakhlagh¹, Amir Manbachi^{1

2

5}, Mehmet Remzi Dokmeci^{1

2}, Shaochen Chen^{6

7

8}, Su Ryon Shin^{1

2}, Yu Shrike Zhang^{1

2}, Ali Khademhosseini^{1

2

9

10

11

12

13}

Affiliations

¹ Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA.
² Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
³ Microoptics and GRIN Optics Group, Applied Physics Department, Faculty of Physics, University of Santiago de Compostela, Santiago de Compostela, 15782, Spain.
⁴ Polymeric Materials Research Group, Department of Materials Science and Engineering, Sharif University of Technology, Tehran, 1458889694, Iran.
⁵ Department of Biomedical Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
⁶ Department of NanoEngineering, University of California, San Diego, La Jolla, CA, 92093, USA.
⁷ Department of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, USA.
⁸ Materials Science and Engineering Program, University of California, San Diego, La Jolla, CA, 92093, USA.
⁹ Center for Minimally Invasive Therapeutics (C-MIT), University of California-Los Angeles, Los Angeles, CA, 90095, USA.
¹⁰ Department of Radiology, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA, 90095, USA.
¹¹ Department of Bioengineering, Department of Chemical and Biomolecular Engineering, Henry Samueli School of Engineering and Applied Sciences, University of California-Los Angeles, Los Angeles, CA, 90095, USA.
¹² California NanoSystems Institute (CNSI), University of California-Los Angeles, Los Angeles, CA, 90095, USA.
¹³ Department of Bioindustrial Technologies, Konkuk University, Seoul, 143-701, Republic of Korea.

Abstract

A stereolithography-based bioprinting platform for multimaterial fabrication of heterogeneous hydrogel constructs is presented. Dynamic patterning by a digital micromirror device, synchronized by a moving stage and a microfluidic device containing four on/off pneumatic valves, is used to create 3D constructs. The novel microfluidic device is capable of fast switching between different (cell-loaded) hydrogel bioinks, to achieve layer-by-layer multimaterial bioprinting. Compared to conventional stereolithography-based bioprinters, the system provides the unique advantage of multimaterial fabrication capability at high spatial resolution. To demonstrate the multimaterial capacity of this system, a variety of hydrogel constructs are generated, including those based on poly(ethylene glycol) diacrylate (PEGDA) and gelatin methacryloyl (GelMA). The biocompatibility of this system is validated by introducing cell-laden GelMA into the microfluidic device and fabricating cellularized constructs. A pattern of a PEGDA frame and three different concentrations of GelMA, loaded with vascular endothelial growth factor, are further assessed for its neovascularization potential in a rat model. The proposed system provides a robust platform for bioprinting of high-fidelity multimaterial microstructures on demand for applications in tissue engineering, regenerative medicine, and biosensing, which are otherwise not readily achievable at high speed with conventional stereolithographic biofabrication platforms.

Keywords: bioprinting; digital light prototyping; digital micromirror devices; microfluidics; multimaterials.

MeSH terms

Animals
Bioprinting
Hydrogel, Polyethylene Glycol Dimethacrylate
Microfluidics*
Rats
Tissue Engineering
Tissue Scaffolds
Vascular Endothelial Growth Factor A

Substances

Vascular Endothelial Growth Factor A
Hydrogel, Polyethylene Glycol Dimethacrylate

Abstract

MeSH terms

Substances

Grants and funding