Phenotypic Screening of Small Molecules with Antimalarial Activity for Three Different Parasitic Life Stages

Nobutaka Kato; Sandra March; Sangeeta N Bhatia; Matthias Marti

doi:10.1007/978-1-4939-7847-2_3

Phenotypic Screening of Small Molecules with Antimalarial Activity for Three Different Parasitic Life Stages

Methods Mol Biol. 2018:1787:41-52. doi: 10.1007/978-1-4939-7847-2_3.

Authors

Nobutaka Kato¹, Sandra March^{2

3

4

5}, Sangeeta N Bhatia^{2

3

4

5}, Matthias Marti^{6

7}

Affiliations

¹ Broad Institute of MIT and Harvard, Cambridge, MA, USA. nkato@broadinstitute.org.
² Broad Institute of MIT and Harvard, Cambridge, MA, USA.
³ Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, USA.
⁴ Howard Hughes Medical Institute, Cambridge, MA, USA.
⁵ Koch Institute for Integrative Cancer Research, Cambridge, MA, USA.
⁶ Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow, UK.
⁷ Department of Immunology and Infectious Diseases, Harvard TH Chan School of Public Health, Boston, MA, USA.

PMID: 29736708
DOI: 10.1007/978-1-4939-7847-2_3

Abstract

Malaria remains one of the deadliest infectious diseases globally. Available therapeutic agents are already limited in their efficacy, and drug resistance threatens to diminish further our ability to prevent and treat the disease. Despite a renewed effort to identify compounds with antimalarial activity, the drug discovery and development pipeline lacks target diversity and availability of compounds that target liver- and gametocyte-stage parasites. Phenotypic screens are a powerful and valuable tool for identifying new chemical compounds with antimalarial activity. This chapter highlights recent phenotypic screening methodologies for all three parasitic life stages.

Keywords: Asexual blood-stage; Gametocyte-stage; Liver-stage; Malaria; Phenotypic screening; Plasmodium falciparum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antimalarials / pharmacology*
Drug Discovery*
Erythrocytes / drug effects
Erythrocytes / parasitology
Humans
Life Cycle Stages / drug effects*
Liver / drug effects
Liver / parasitology
Phenotype
Plasmodium / drug effects*
Plasmodium / physiology*
Small Molecule Libraries*

Substances

Antimalarials
Small Molecule Libraries

Grants and funding

HHMI/Howard Hughes Medical Institute/United States