RNA Binding Protein RNPC1 Inhibits Breast Cancer Cell Metastasis via Activating STARD13-Correlated ceRNA Network

Lufeng Zheng; Zhiting Zhang; Shufang Zhang; Qianqian Guo; Feng Zhang; Lanlan Gao; Haiwei Ni; Xinwei Guo; Chenxi Xiang; Tao Xi

doi:10.1021/acs.molpharmaceut.7b01123

RNA Binding Protein RNPC1 Inhibits Breast Cancer Cell Metastasis via Activating STARD13-Correlated ceRNA Network

Mol Pharm. 2018 Jun 4;15(6):2123-2132. doi: 10.1021/acs.molpharmaceut.7b01123. Epub 2018 May 15.

Authors

Lufeng Zheng^{1

2}, Zhiting Zhang^{1

2}, Shufang Zhang^{1

2}, Qianqian Guo^{1

2}, Feng Zhang^{1

2}, Lanlan Gao^{1

2}, Haiwei Ni^{1

2}, Xinwei Guo^{1

2}, Chenxi Xiang^{1

2}, Tao Xi^{1

2}

Affiliations

¹ School of Life Science and Technology , China Pharmaceutical University , Nanjing 210009 , People's Republic of China.
² Jiangsu Key Laboratory of Carcinogenesis and Intervention , China Pharmaceutical University , Nanjing 210009 , People's Republic of China.

PMID: 29733656
DOI: 10.1021/acs.molpharmaceut.7b01123

Abstract

RNA binding proteins (RBPs) are pivotal post-transcriptional regulators. RNPC1, an RBP, acts as a tumor suppressor through binding and regulating the expression of target genes in cancer cells. This study disclosed that RNPC1 expression was positively correlated with breast cancer patients' relapse-free and overall survival and that RNPC1 suppressed breast cancer cell metastasis. Mechanistically, RNPC1 promotes competing endogenous RNA (ceRNA) network crosstalk among STARD13, CDH5, HOXD10, and HOXD1 (STARD13-correlated ceRNA network), which we previously confirmed in breast cancer cells through stabilizing the transcripts and thus facilitating the expression of these four genes in breast cancer cells. Furthermore, RNPC1 overexpression restrained the promotion of STARD13, CDH5, HOXD10, and HOXD1 knockdown on cell metastasis. Notably, RNPC1 expression was positively correlated with CDH5, HOXD1, and HOXD10 expression in breast cancer tissues and attenuated adriamycin resistance. Taken together, these results identified that RNPC1 could inhibit breast cancer cell metastasis via promoting a STARD13-correlated ceRNA network.

Keywords: RNPC1; STARD13; breast cancer metastasis; ceRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions / genetics
Breast Neoplasms / genetics*
Breast Neoplasms / pathology
Female
GTPase-Activating Proteins / genetics*
GTPase-Activating Proteins / metabolism
Gene Expression Regulation, Neoplastic*
Gene Knockdown Techniques
Gene Regulatory Networks
HEK293 Cells
Humans
MCF-7 Cells
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism*
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism*
Tumor Suppressor Proteins / genetics*
Tumor Suppressor Proteins / metabolism

Substances

3' Untranslated Regions
GTPase-Activating Proteins
RBM38 protein, human
RNA, Long Noncoding
RNA-Binding Proteins
STARD13 protein, human
Tumor Suppressor Proteins