Objective: To investigate the value of circulating miR-152 in the early prediction of postoperative biochemical recurrence of prostate cancer.
Methods: Sixty-six cases of prostate cancer were included in this study, 35 with and 31 without biochemical recurrence within two years postoperatively, and another 31 healthy individuals were enrolled as normal controls. The relative expression levels of circulating miR-152 in the serum of the subjects were detected by qRT-PCR, its value in the early diagnosis of postoperative biochemical recurrence of prostate cancer was assessed by ROC curve analysis, and the correlation of its expression level with the clinicopathological parameters of the patients were analyzed.
Results: The expression of circulating miR-152 was significantly lower in the serum of the prostate cancer patients than in the normal controls (t = －5.212, P = 0.001), and so was it in the patients with than in those without postoperative biochemical recurrence (t = －5.727, P = 0.001). The ROC curve for the value of miR-152 in the early prediction of postoperative biochemical recurrence of prostate cancer showed the area under the curve (AUC) to be 0.906 (95% CI: 0.809－0.964), with a sensitivity of 91.4% and a specificity of 80.6%. The expression level of miR-152 was correlated with the Gleason score, clinical stage of prostate cancer, biochemical recurrence, and bone metastasis (P <0.05), decreasing with increased Gleason scores and elevated clinical stage of the malignancy. No correlation, however, was found between the miR-152 expression and the patients' age or preoperative PSA level (P >0.05).
Conclusions: The expression level of circulating miR-152 is significantly reduced in prostate cancer patients with biochemical recurrence after prostatectomy and could be a biomarker in the early prediction of postoperative biochemical recurrence of the malignancy.
目的: 探讨循环微小RNA 152(miR-152)对前列腺癌生化复发早期预测的价值。方法: 收集将要进行前列腺癌手术的患者66例（生化复发组：35例，术后2年内生化复发；未生化复发组：31例，术后2年内未生化复发），另择年龄相匹配的31例健康男性作为健康对照组。用qRT-PCR法检测前列腺癌患者和健康对照组的循环miR-152表达水平。应用ROC曲线分析循环miR-152对前列腺癌早期生化复发的诊断价值。分析复发组循环miR-152表达水平与临床病理参数（年龄、术前PSA水平、Gleason积分、临床分期、骨转移）之间的相关性。结果: 循环miR-152在前列腺癌术后患者（未生化复发及生化复发）与健康对照组的表达差异有统计学意义（t=－5.212，P=0.001）。前列腺癌术后生化复发组患者循环miR-152的表达量较未生化复发组患者低，差异有统计学意义（t=－5.727，P=0.001）。循环miR-152对前列腺癌术后生化复发早期预测的ROC曲线下面积（AUC）为0.906（95％CI,0.809~0.964），灵敏度和特异度分别为91.4％、 80.6％。循环miR-152表达水平与Gleason积分、临床分期、有无生化复发和骨转移相关（P均<0.05）；随着Gleason积分、临床分期的增加，循环miR-152的表达水平下降。但miR-152表达量与患者年龄、术前PSA无关（P均>0.05）。结论： 循环miR-152表达水平在前列腺癌术后生化复发患者中显著降低，可能成为前列腺癌生化复发早期预测的分子标志物。.
Keywords: biochemical recurrence; miR-152; serum PSA; prostate cancer.