Concomitant desalting and concentration of neuropeptides on a donut-shaped surface pattern for MALDI mass spectrometry

Chem Commun (Camb). 2018 May 31;54(45):5688-5691. doi: 10.1039/c8cc02168f.

Abstract

We demonstrate that a donut-shaped surface pattern consisting of a central hydrophobic region and a surrounding hydrophilic region simultaneously concentrates and desalts a solution of neuropeptides with a high salt content. Our approach greatly simplifies the sample preparation process for MALDI mass spectrometry.

MeSH terms

  • Angiotensin I / chemistry
  • Bradykinin / analogs & derivatives
  • Bradykinin / chemistry
  • Corticotropin-Like Intermediate Lobe Peptide / chemistry
  • Gold / chemistry
  • Hydrophobic and Hydrophilic Interactions
  • Kinetics
  • Limit of Detection
  • Models, Chemical
  • Neuropeptides / chemistry*
  • Saline Waters / chemistry*
  • Silicon / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Sulfhydryl Compounds / chemistry

Substances

  • Neuropeptides
  • Sulfhydryl Compounds
  • bradykinin, des-Arg(9)-
  • n-octadecyl mercaptan
  • Corticotropin-Like Intermediate Lobe Peptide
  • Gold
  • Angiotensin I
  • Bradykinin
  • Silicon