This study aims to explore the vascularization of hydroxyapatite/tricalcium phosphate(HA/TCP)biomaterials implanted in mice during osteoinduction. The HA/TCP biomaterials were implanted in muscle of mice, and2, 4, 6, 8, 10 and 12 weeks after the implantation, the materials were harvested to prepare serial sections and hematoxylineosin(HE) staining. The process of vascularization was dynamically described, and the area percentage of neovascularization was quantitatively analyzed. The results showed that neovascularization formation was a continuous and dynamic process. The neovascularization appeared largely in the first two weeks, with a rising trend in week 4,reached peak in week 6, and gradually reduced in week 8. The results provide ideas for improving the success rate of bone tissue engineering, and indicate the mechanism of osteoinduction.
本研究旨在探讨羟基磷灰石/磷酸三钙（HA/TCP）生物陶瓷材料植入小鼠体内骨诱导过程中新生血管的形成过程。将 HA/TCP 材料植入小鼠肌肉中，手术后 2、4、6、8、10 和 12 周，收获材料制备连续切片，通过观察 HE 染色图片动态描述新生血管形成过程，并定量分析新生血管形成百分比。结果显示新生血管形成是一个连续、动态的过程，其在第 2 周时就大量生成，4 周时呈增长趋势，6 周时达到峰值，8 周后逐步减少。本研究结果为提高骨组织工程成功率提供了思路，也从血管化方面揭示了骨诱导形成的机制。