Functionalized DNA: A New Replicable Biopolymer

Oliver Thum; Stefan Jäger; Michael Famulok

doi:10.1002/1521-3773(20011105)40:21<3990::AID-ANIE3990>3.0.CO;2-O

Functionalized DNA: A New Replicable Biopolymer

Angew Chem Int Ed Engl. 2001 Nov 5;40(21):3990-3993. doi: 10.1002/1521-3773(20011105)40:21<3990::AID-ANIE3990>3.0.CO;2-O.

Authors

Oliver Thum¹, Stefan Jäger¹, Michael Famulok¹

Affiliation

¹ Kekulé-Institut für Organische Chemie und Biochemie Universität Bonn Gerhard-Domagk-Strasse 1, 53121 Bonn (Germany) Fax: (+49) 228-735388.

PMID: 29712240
DOI: 10.1002/1521-3773(20011105)40:21<3990::AID-ANIE3990>3.0.CO;2-O

Abstract

New DNA: By enzymatic polymerization of base-modified nucleoside triphosphates, a functionalized DNA (fDNA; see picture) was generated in which every base bears an additional amino acid like residue, thus mimicking the functional group repertoire of peptides on a nucleic acid backbone. These modified oligonucleotides can in turn serve as templates for polymerase chain reaction amplification, thus utilizing fDNA as a novel class of biopolymers for in vitro selection techniques.

Keywords: combinatorial chemistry; in vitro selection; nucleotides; oligonucleotides.