Polysaccharides from Diaphragma juglandis fructus: Extraction optimization, antitumor, and immune-enhancement effects

Qingran Meng; Yaqi Wang; Feng Chen; Tiancun Xiao; Lianfu Zhang

doi:10.1016/j.ijbiomac.2018.04.121

Polysaccharides from Diaphragma juglandis fructus: Extraction optimization, antitumor, and immune-enhancement effects

Int J Biol Macromol. 2018 Aug:115:835-845. doi: 10.1016/j.ijbiomac.2018.04.121. Epub 2018 Apr 27.

Authors

Qingran Meng¹, Yaqi Wang², Feng Chen², Tiancun Xiao³, Lianfu Zhang⁴

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, PR China; Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, SC 29634, USA.
² Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, SC 29634, USA.
³ Inorganic Chemistry Laboratory, University of Oxford, Oxford OX1 3QR, UK.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, PR China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan, University, Wuxi, Jiangsu 214122, PR China. Electronic address: lianfu@jiangnan.edu.cn.

PMID: 29705107
DOI: 10.1016/j.ijbiomac.2018.04.121

Abstract

This study discusses the optimization of the microwave-assisted extraction of polysaccharides from Diaphragma juglandis (DJPs). One main fraction (DJP-2) was successfully purified by ion-exchange chromatography and gel-permeation chromatography. The results showed that the optimal extraction conditions with DJP extraction yield of 4.7 ± 0.28% were water to raw material ratio of 20 mL/g, extraction time of 40 min, and microwave extraction power of 400 W. Bioassays indicated that DJP-2 could effectively suppress the proliferation of HepG2 and BGC-82 cell lines. In addition, DJP-2 could significantly enhance phagocytosis; stimulate the production of NO, tumor necrosis factor-α (TNF-α), and interleukins (IL-6 and IL-1β); and promote their corresponding mRNA expression levels in a dose-dependent manner. Meanwhile, CR3, MR, and TLR2 were confirmed to be the major membrane receptors of DJP-2 on RAW 264.7. All these results indicated that DJP-2 could be a potential antitumor and immunomodulatory agent in the field of pharmacology.

Keywords: Antitumor activity; Diaphragma juglandis fructus; Immunomodulatory activity; Polysaccharides.

MeSH terms

Animals
Antineoplastic Agents / chemistry
Antineoplastic Agents / isolation & purification*
Antineoplastic Agents / pharmacology
Cell Proliferation / drug effects
Chlorophyta / chemistry*
Cytokines / metabolism
Hep G2 Cells
Humans
Immunologic Factors / chemistry
Immunologic Factors / isolation & purification*
Immunologic Factors / pharmacology*
Mice
Nitric Oxide / metabolism
Phagocytosis / drug effects
Polysaccharides / chemistry
Polysaccharides / isolation & purification*
Polysaccharides / pharmacology*
RAW 264.7 Cells
Water / chemistry

Substances

Antineoplastic Agents
Cytokines
Immunologic Factors
Polysaccharides
Water
Nitric Oxide