Physiological and proteomic analyses of coix seed aging during storage

Abstract

Although a series of physio-biochemical changes of coix seed occur during the storage, the underlying mechanism remains unknown. The present study aimed to investigate the aging mechanism of coix seed during storage. Proteome patterns of coix seed stored for 1-month, 5-month and 10-month at room temperature were compared using 2-dimensional gel electrophoresis (2-DE) and mass spectra. Thirty-one differentially expressed proteins (DEPs) were detected, which involved seven pathways including starch and sucrose metabolism, carbon metabolism, RNA transport, proteasome, protein processing in endoplasmic reticulum, ribosome, and RNA degradation. Sucrose synthase 1 was associated with sucrose metabolism and affected the sucrose content during the storage. Increased ambient temperature enhanced respiration of coix after 5-month storage, and overexpression of aconitate hydratase 2 promoted the generation of energy. In addition, the proteins involved in the antioxidant system and resistance stimulus of coix seed were mostly up-regulated during storage.

Keywords: 2-DE; Acetonitrile (PubChem CID: 6342); Aging; Benzene (PubChem CID: 241); Coix seed; Coomassie Brilliant Blue G 250 (PubChem CID: 61364); Ellman’s Reagent (PubChem CID: 6254); Iodoacetamide (PubChem CID: 3727); N,N,N′,N′-Tetramethylethylenediamine (PubChem CID: 8037); Polyviny-lpolypyrrolidone (PubChem CID: 6917); Proteomics; Storage; Sulfanyl (PubChem CID: 5460613); Trifluoroacetic acid (PubChem CID: 6422); Trihydroxymethyl aminomethane (PubChem CID: 6503).