Non-invasive microsensing technique has been widely used in evaluating the adaptive responses of plant cells and tissues to abiotic stresses. One of the representative techniques is the microelectrode ion flux estimation (MIFE), which allows concurrent quantification of net fluxes with high spatial and temporal resolution. More importantly, this technique permits simultaneous recording of ion concentration and mobility with less intervention to the in situ physiological status. With the availability of such advanced technique, the last three decades have seen a significant progress towards the role of ion signaling in a variety of abiotic stresses including salinity, extreme temperature, osmotic stress, hypoxia, and drought. In this review, we gave a brief introduction of the MIFE working principles and focused on its applications in detecting ion responses to various abiotic stresses.
非损伤性微测技术已成为研究植物细胞和组织对各种非生物逆境适应性反应的普遍手段,该技术具有非损伤性、高时间分辨率和空间分辨率等特点,能够保持被测样品的完整性,在相对真实的生理环境状态下,测得进出样品细胞膜的离子浓度、流速和运动方向等参数.过去近30年间,很多研究通过微电极离子流技术(microelectrode ion flux estimation,MIFE)或者类似的非损伤性微测技术阐释了离子跨膜转运过程对植物应对生物逆境胁迫(盐害、涝害、冷害、干旱、热害等)的响应机理,在生命科学领域做出了重要贡献.本文以MIFE技术为例,较为详细地阐述了其工作原理以及该技术与其他技术的结合在作物逆境应答中的信号转导研究进展.
Keywords: abiotic stress; crop; ion flux; microelectrode ion flux estimation.