Hamuramicins A and B, 22-membered macrolides, produced by an endophytic actinomycete Allostreptomyces sp. K12-0794

Takuya Suga; Tōru Kimura; Yuki Inahashi; Masato Iwatsuki; Kenichi Nonaka; Akira Také; Atsuko Matsumoto; Yōko Takahashi; Satoshi Ōmura; Takuji Nakashima

doi:10.1038/s41429-018-0055-x

Hamuramicins A and B, 22-membered macrolides, produced by an endophytic actinomycete Allostreptomyces sp. K12-0794

J Antibiot (Tokyo). 2018 Jul;71(7):619-625. doi: 10.1038/s41429-018-0055-x. Epub 2018 Apr 24.

Authors

Takuya Suga^{1

2}, Tōru Kimura³, Yuki Inahashi^{4

3}, Masato Iwatsuki^{4

3}, Kenichi Nonaka^{4

3}, Akira Také⁴, Atsuko Matsumoto^{4

3}, Yōko Takahashi⁴, Satoshi Ōmura⁴, Takuji Nakashima^{5

6}

Affiliations

¹ Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Tokyo, 108-8641, Japan. di13003@st.kitasato-u.ac.jp.
² Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Tokyo, 108-8641, Japan. di13003@st.kitasato-u.ac.jp.
³ Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Tokyo, 108-8641, Japan.
⁴ Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Tokyo, 108-8641, Japan.
⁵ Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Tokyo, 108-8641, Japan. takuji@lisci.kitasato-u.ac.jp.
⁶ Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Tokyo, 108-8641, Japan. takuji@lisci.kitasato-u.ac.jp.

PMID: 29691484
DOI: 10.1038/s41429-018-0055-x

Abstract

Two new compounds, designated as hamuramicins A (1) and B (2), were isolated from the cultured broth of an endophytic actinomycete Allostreptomyces sp. K12-0794 by silica gel column chromatography and HPLC. The structures of 1 and 2 were elucidated as 22-membered macrolide containing triene and trienone with an alkyl side chain by spectroscopic analyses including NMR experiments. Both compounds showed growth inhibition activity against Kocuria rhizophia and Xanthomonas oryzae pv. oryzae as well as human cell line toxicity.

MeSH terms

Actinomycetaceae / drug effects
Actinomycetaceae / metabolism*
Anti-Bacterial Agents / biosynthesis*
Anti-Bacterial Agents / pharmacology*
Antibiotics, Antineoplastic / biosynthesis
Antibiotics, Antineoplastic / pharmacology
Bacteria / drug effects
Cell Line, Tumor
Chromatography, High Pressure Liquid
Endophytes
Fermentation
Humans
Macrolides / isolation & purification*
Macrolides / pharmacology*
Magnetic Resonance Spectroscopy
Microbial Sensitivity Tests

Substances

Anti-Bacterial Agents
Antibiotics, Antineoplastic
Macrolides